High-throughput production of human proteins for crystallization: The SGC experience.
Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O
J Struct Biol. 2010 Jun 10. ():. PubMed Article
Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O
J Struct Biol. 2010 Jun 10. ():. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 26103 | pNIC28-Bsa4 | SGC Empty backbone for bacterial expression |
| 26105 | pNIC-CTHF | SGC Empty backbone for bacterial expression |
| 26106 | pNH-TrxT | SGC Empty backbone for bacterial expression |
| 26107 | pNIC-ZB | SGC Empty backbone for bacterial expression |
| 26108 | pFB-LIC-Bse | SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection |
| 26242 | BL21(DE3)-R3-pRARE2 | Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes. |