High-throughput production of human proteins for crystallization: the SGC experience.

Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O
J Struct Biol. 2010 Oct;172(1):3-13. doi: 10.1016/j.jsb.2010.06.008. Epub 2010 Jun 10. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
26103	pNIC28-Bsa4	SGC Empty backbone for bacterial expression
26105	pNIC-CTHF	SGC Empty backbone for bacterial expression
26106	pNH-TrxT	SGC Empty backbone for bacterial expression
26107	pNIC-ZB	SGC Empty backbone for bacterial expression
26108	pFB-LIC-Bse	SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
26242	BL21(DE3)-R3-pRARE2	Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.