A versatile viral system for expression and depletion of proteins in mammalian cells.
Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.
PLoS One. 2009 Aug 6;4(8):e6529. PubMed Article
Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.
PLoS One. 2009 Aug 6;4(8):e6529. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 17291 | pLenti CMV/TO Hygro DEST (693-2) | 3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro |
| 17292 | pLenti CMV/TO Neo DEST (685-3) | 3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo |
| 17293 | pLenti CMV/TO Puro DEST (670-1) | 3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro |
| 17294 | pLenti CMV/TO Zeo DEST (644-1) | 3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Zeo |
| 17295 | pLenti X2 Hygro DEST (w17-1) | 3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Hygro |
| 17296 | pLenti X2 Puro DEST (w16-1) | 3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro |
| 17297 | pLenti X1 Puro DEST (694-6) | 3rd gen lentiviral promoter-less Gateway destination vector, Puro |
| 17338 | pENTR/pSUPER+ (575-1) | Gateway entry vector for shRNA expression under H1 promoter |
| 17386 | pQCXP CMV/TO DEST (w360-1) | Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro |
| 17387 | pENTR/pSM2(U6) (w51-1) | Gateway entry vector for miRNA expression under U6 promoter |
| 17388 | pENTR/pSM2(CMV/TO) (w55-1) | Gateway entry vector for miRNA expression under CMV/TO promoter |
| 17389 | pENTR/pSM2(CMV) (w57-1) | Gateway entry vector for miRNA expression under CMV promoter |
| 17390 | pLenti X2 Neo DEST (w18-1) | 3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo |
| 17391 | pLenti X2 Blast DEST (w46-2) | 3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Blast |
| 17392 | pLenti CMV Neo DEST (705-1) | 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo |
| 17393 | pQCXP CMV/TO empty (w368-1) | Retroviral Empty control vector, CMV/TO promoter, Puro |
| 17394 | pQCXIH CMV/TO DEST (w382-1) | Retroviral Gateway destination vector, Expression, CMV/TO promoter, Hygro |
| 17396 | pENTR4-GFP-C1 (w392-1) | Gateway entry vector for N-term eGFP fusion, frame C1 |
| 17397 | pENTR4-GFP-C3 (w393-1) | Gateway entry vector for N-term eGFP fusion, frame C3 |
| 17398 | pENTR1A no ccDB (w48-1) | Gateway entry 1A vector, no ccDB gene |
| 17399 | pQCXIN X2 DEST (w310-1) | Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Neo |
| 17400 | pQCXIB CMV/TO DEST (w320-1) | Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast |
| 17401 | pQCXIZ CMV/TO DEST (w321-2) | Retroviral Gateway destination vector, Expression, CMV/TO promoter, Zeo |
| 17402 | pENTR1A-NTAP-A (w322-1) | Gateway entry vector for NTAP (N-terminal affinity purification), frame A |
| 17403 | pENTR1A-NTAP-B (w323-1) | Gateway entry vector for NTAP (N-terminal affinity purification), frame B |
| 17404 | pENTR1A-NTAP-C (w324-1) | Gateway entry vector for NTAP (N-terminal affinity purification), frame C |
| 17407 | pENTR4-V5-2-Ubc9 (w345-2) | Gateway entry vector for N-term V5 tag, frame B and Ubc9 C-term |
| 17413 | pENTR1A-FKBP12DD C-term (w350-2) | Gateway entry vector for Degron, C-term |
| 17414 | pENTR4-FKBP12DD N-term A (B3-1) | Gateway entry vector for Degron, N-term, frame A |
| 17415 | pENTR4-FKBP12DD N-term B (w385-4) | Gateway entry vector for Degron, N-term, frame B |
| 17416 | pENTR4-FKBP12DD N-term C (w385-10) | Gateway entry vector for Degron, N-term, frame C |
| 17423 | pENTR4-FLAG (w210-2) | Gateway entry vector for N-term FLAG tag |
| 17424 | pENTR4 no ccDB (686-1) | Gateway entry vector, no ccDB gene |
| 17425 | pENTR4-V5 (w71-3) | Gateway entry vector for N-term V5 tag, frame A |
| 17426 | pENTR4-V5-2 (w234-1) | Gateway entry vector for N-term V5 tag, frame B |
| 17427 | pEF-ENTR A (696-6) | Gateway entry vector with EF1-a promoter, V5-His C-term, frame A |
| 17428 | pEF-ENTR B (708-1) | Gateway entry vector with EF1-a promoter, V5-His C-term, frame B |
| 17429 | pEF-ENTR C (709-1) | Gateway entry vector with EF1-a promoter, V5-His C-term, frame C |
| 17430 | pEF-ENTR-LacZ (710-1) | Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z |
| 17431 | pLenti CMV/TO GFP-Zeo DEST (719-1) | 3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo |
| 17432 | pLenti CMV/TO DEST (775-1) | 3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter |
| 17446 | pLenti CMV GFP Hygro (656-4) | 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro |
| 17447 | pLenti CMV GFP Neo (657-2) | 3rd gen lentiviral eGFP expression vector, CMV promoter, Neo |
| 17448 | pLenti CMV GFP Puro (658-5) | 3rd gen lentiviral eGFP expression vector, CMV promoter, Puro |
| 17449 | pLenti CMV GFP Zeo (637-7) | 3rd gen lentiviral eGFP expression vector, CMV promoter, Zeo |
| 17451 | pLenti CMV Blast DEST (706-1) | 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast |
| 17452 | pLenti CMV Puro DEST (w118-1) | 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro |
| 17453 | pENTR/pTER+ (430-1) | Gateway entry vector for shRNA expression under H1/TO promoter |
| 17454 | pLenti CMV Hygro DEST (w117-1) | 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro |
| 17455 | pL106P-LUC-ENTR1A(w451-1) | Gateway entry vector, Degron N-term Luciferase |
| 17470 | pENTR/pTER shEGFP #1 (628-2) | Gateway entry vector for EGFPshRNA#1 under H1/TO promoter |
| 17471 | pENTR/pTER shEGFP #2 (728-2) | Gateway entry vector for EGFPshRNA#2 under H1/TO promoter |
| 17472 | pENTR/pTER shLUC (w177-1) | Gateway entry vector for Firefly Luciferase shRNA under H1/TO promoter |
| 17473 | pENTR-LUC (w158-1) | Gateway entry vector, Firefly Luciferase |
| 17474 | pQCXIP X2 DEST (w270-1) | Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Puro |
| 17475 | pQCXIB CMV/TO LUC (w431-1) | Retroviral Luciferase Expression vector, CMV/TO promoter, Blast |
| 17476 | pQCXP CMV/TO LUC (w432-1) | Retroviral Luciferase expression vector, CMV/TO promoter, Puro |
| 17477 | pLenti CMV Puro LUC (w168-1) | 3rd gen lentiviral Luciferase expression vector, CMV promoter, Puro |
| 17478 | pLenti X2 Neo empty (w36-1) | 3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Neo |
| 17479 | pLenti X2 Neo/pTER shEGFP#1 (w21-2) | 3rd gen lentiviral eGFP shRNA #1 (H1/TO promoter), 3’LTR insertion, Neo |
| 17480 | pLenti X2 Neo/pTER shEGFP#2 (w22-2) | 3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo |
| 17481 | pLenti CMV/TO eGFP Puro (w159-1) | 3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro |
| 17482 | pLenti CMV/TO Puro empty (w175-1) | 3rd gen lentiviral empty control vector, CMV/TO promoter, Puro |
| 17483 | pLenti X2 Neo/pTER shLUC (w181-1) | 3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo |
| 17484 | pLenti CMV/TO Hygro empty (w214-1) | 3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro |
| 17485 | pLenti CMV/TO Neo empty (w215-1) | 3rd gen lentiviral empty control vector, CMV/TO promoter, Neo |
| 17486 | pLenti CMV Blast empty (w263-1) | 3rd gen lentiviral empty control vector, CMV promoter, Blast |
| 17487 | pQCXIB empty (w335-1) | Retroviral Empty control vector, CMV/TO promoter, Blast |
| 17488 | pQCXIN X2/pSM2(CMV/TO) empty (w334-1) | Retroviral Empty control vector, miRNA (CMV/TO), 3’LTR insertion, Neo |
| 17489 | pQCXIN X2/pTER shLUC (w347-1) | Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo |
| 17491 | pQCXIP X2/pTER shLUC (w454-1) | Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro |
| 17492 | pLenti CMV TetR Blast (716-1) | 3rd gen lentiviral Tetracycline repressor expression vector, CMV promoter, Blast |
| 17740 | pQCXP CMV/TO V5-LUC (w476-1) | Retroviral V5-Luciferase expression vector, CMV/TO promoter, Puro |
| 17741 | pENTR4-GST 6P-1 (w487-1) | Gateway entry vector for N-term GST fusion, frame 6P-1 |
| 19065 | pLenti PGK Blast DEST (w524-1) | 3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast |
| 19066 | pLenti PGK Hygro DEST (w530-1) | 3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro |
| 19067 | pLenti PGK Neo DEST (w531-1) | 3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo |
| 19068 | pLenti PGK Puro DEST (w529-2) | 3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro |
| 19069 | pLenti PGK GFP Blast (w510-5) | 3rd gen lentiviral eGFP expression vector, PGK promoter, Blast |
| 19070 | pLenti PGK GFP Puro (w509-5) | 3rd gen lentiviral eGFP expression vector, PGK promoter, Puro |
| 19135 | pENTR4-V5-LUC (w468-1) | Gateway entry vector, V5-Luciferase |
| 19166 | pLenti PGK Blast V5-LUC (w528-1) | 3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast |
| 19167 | pQCXIN X2/pTER shEGFP#1 (w491-1) | Retroviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo |
| 19170 | pENTR/pSM2(CMV) GFP (w513-1) | Gateway entry vector for miRNA expression under CMV promoter and GFP reporter |
| 19360 | pLenti PGK V5-LUC Puro (w543-1) | 3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro |
| 19364 | pENTR1A-GFP-N2 (FR1) | Gateway entry vector for C-term eGFP fusion, frame N2 |
| 19732 | pLenti CMV GFP DEST (736-1) | 3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression |
| 19785 | pLenti CMV/TO V5-LUC Puro (w549-1) | 3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro |
| 20025 | pENTR4-HA-myc-nuc (w557-1) | Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags) |
| 20026 | pENTR4-myc-nuc (w556-1) | Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags) |