A toolkit and robust pipeline for the generation of fosmid-based reporter genes in C. elegans.
Tursun B, Cochella L, Carrera I, Hobert O
PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 2009 Mar 4. PubMed Article
Tursun B, Cochella L, Carrera I, Hobert O
PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 2009 Mar 4. PubMed Article
Plasmids from Article
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69316 | pBALU1 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp |
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69317 | pBALU2 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of yfp |
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69318 | pBALU3 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of cfp |
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69319 | pBALU4 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of mChO (mCherry variant) |
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69321 | pBALU6 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: Venus in frame w/ FRT-galK-FRT; no introns; N-terminal fusion |
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69324 | pBALU9 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP |
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69328 | pBALU12 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOP |
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69334 | pBALU18 | Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2ndmChO intron; use C-term after STOP |
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69339 | pBALU-ext | It allows to extend the genomic insert in a fosmid. |
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