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The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.

Stewart GS, Panier S, Townsend K, Al-Hakim AK, Kolas NK, Miller ES, Nakada S, Ylanko J, Olivarius S, Mendez M, Oldreive C, Wildenhain J, Tagliaferro A, Pelletier L, Taubenheim N, Durandy A, Byrd PJ, Stankovic T, Taylor AM, Durocher D
Cell. 2009 Feb 6;136(3):420-34. doi: 10.1016/j.cell.2008.12.042. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
133976pcDNA3-Flag-RNF168mammalian expression vector of Flag tagged wild type RNF168
133977pcDNA3-eGFP-NLS-RNF168mammalian expression vector of eGFP tagged wild type RNF168
133978pcDNA3-Flag-RNF168 delta MIU1mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted
133979pcDNA3-Flag-RNF168 delta MIU2mammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deleted
133980pcDNA5-eGFP-FRT-T0-RNF168 delta MIU1/MIU2inducible mammalian expression vector of eGFP tagged RNF168 where both motifs interacting with ubiquitin have been deleted
133981pcDNA3-Flag-RNF168 delta MIU1/MIU2mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted
133982pcDNA3-Flag-RNF168 delta RINGmammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)
133983pcDNA3-eGFP-NLS-RNF168 delta RINGmammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

Antibodies from Article