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The tumor suppressor CYLD regulates entry into mitosis.
Stegmeier F, Sowa ME, Nalepa G, Gygi SP, Harper JW, Elledge SJ
Proc Natl Acad Sci U S A. 2007 May 22. 104(21):8869-74.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
15506HA-CYLD-WT
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60027HA-CYLD-C601AHA-tagged CYLD catalytic inactive (C601A) expression
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60028GFP-CYLD-C601AN-terminal GFP tagged catalytic inactive (C601A) expression
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60029pTRE-Tight-FLAG-CYLD-C601ACYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag
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60030pTRE-Tight-FLAG-CYLD-WTWT CYLD under control of Tet promoter with N-terminal FLAG tag
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60031MSCV-TAP(FLAG-HA) CYLDCYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained
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60077GFP-CYLD-STOPN-terminal GFP tagged CYLD with stop codon, driven by CMV promoter
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Antibodies from Article