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mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa.
Choi KH, Schweizer HP
Nat Protoc. 2006;1(1):153-61.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
65022pUC18R6K-mini-Tn7T-GmGmR mini-Tn7 vector, R6K replicon
65024pUC18T-mini-Tn7T-TpTpR mini-Tn7 vector, mobilizable
65026pUC18-mini-Tn7T-Gm-LacZ10GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.
65027pUC18-mini-Tn7T-Gm-LacZ20GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.
65028pUC18-mini-Tn7T-Gm-LacZ30GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.
65030pUC18T-mini-Tn7T-Gm-ecfpGmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria
65031pUC18T-mini-Tn7T-Gm-eyfpGmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria
65032pUC18T-mini-Tn7T-Gm-dsRedExpressGmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria
65033pUC18T-mini-Tn7T-Tp-gfpmut3TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria
65034pUC18T-mini-Tn7T-Tp-ecfpTpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria
65035pUC18T-mini-Tn7T-Tp-eyfpTpR mini-Tn7 vector, mobilizable, for YFP tagging bacteria
65036pUC18T-mini-Tn7T-Tp-dsRedExpressTpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria
65037pUC18T-mini-Tn7T-Zeo-gfpmut3ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria
65038pUC18T-mini-Tn7T-Zeo-ecfpZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteria
65039pUC18T-mini-Tn7T-Zeo-eyfpZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteria
65040pUC18T-mini-Tn7T-Zeo-dsRedExpressZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

Antibodies from Article