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Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli.
Tischer BK, von Einem J, Kaufer B, Osterrieder N
Biotechniques. 2006 Feb;40(2):191-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
41017pEPkan-SPCR template for en passant (two-step Red-mediated) mutagenesis
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60960pBAD-I-SceIPlasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis
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60961pEP-EGFPin PCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesis
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61601pEPkan-S2PCR template for en passant (two-step Red-mediated) mutagenesis
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Antibodies from Article