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PDSM, a motif for phosphorylation-dependent SUMO modification.
Hietakangas V, Anckar J, Blomster HA, Fujimoto M, Palvimo JJ, Nakai A, Sistonen L
Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):45-50. doi: 10.1073/pnas.0503698102. Epub 2005 Dec 21.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
118352hGATA-1 WTThis plasmid expresses human GATA-1 WT.
118353hGATA-1 K137RThis plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.
118354hMEF2A WTThis plasmid expresses human MEF2A-1 WT.
118355hMEF2A K403RThis plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.
118356hHSF4b WTThis plasmid expresses human HSF4b WT.
118357hHSF4b K294RThis plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.
118358Gal4-HSF4b WTThis plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.
118359Gal4-HSF4b K294RThis plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.

Antibodies from Article