Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting.
Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot CH
J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10. PubMed Article
Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot CH
J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 54468 | YFP(1-158)-beta-1 in pcDNAI/Amp | An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced. |
| 54469 | YFP(1-158)-beta-2 in pcDNAI/Amp | An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced. |
| 54470 | YFP(1-158)-beta-5 in pcDNAI/Amp | An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced. |
| 54471 | YFP(159-238)-gamma-1 in pcDNAI/Amp | A carboxyl-terminal YFP fragment was fused to Ggamma-1. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced. |
| 54472 | YFP(159-238)-gamma-2 in pcDNAI/Amp | A carboxyl-terminal YFP fragment was fused to Ggamma-2. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced. |
| 54473 | YFP(159-238)-gamma-7 in pcDNAI/Amp | A carboxyl-terminal YFP fragment was fused to Ggamma-7. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced. |
| 60888 | YFP(1-158) in pcDNAI/Amp | This vector attaches YFP(1-158) to the N-terminus of a protein. |
| 60889 | YFP(159-238) in pcDNAI/Amp | This vector attaches YFP(159-238) to the N-terminus of a protein. |