Cell-free translation reconstituted with purified components.
Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T
Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. PubMed Article
Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T
Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124103 | pQE30-His-AlaRS | Bacterial expression plasmid for PURE system components |
| 124104 | pET16b-His-ArgRS | Bacterial expression plasmid for PURE system components |
| 124105 | pQE30-His-AsnRS | Bacterial expression plasmid for PURE system components |
| 124106 | pET21a-AspRS-His | Bacterial expression plasmid for PURE system components |
| 124107 | pET21a-CysRS-His | Bacterial expression plasmid for PURE system components |
| 124108 | pET21a-GlnRS-His | Bacterial expression plasmid for PURE system components |
| 124109 | pET21a-GluRS-His | Bacterial expression plasmid for PURE system components |
| 124110 | pET21a-GlyRS-His | Bacterial expression plasmid for PURE system components |
| 124111 | pET21a-HisRS-His | Bacterial expression plasmid for PURE system components |
| 124112 | pET21a-His-IleRS | Bacterial expression plasmid for PURE system components |
| 124113 | pET21a-LeuRS-His | Bacterial expression plasmid for PURE system components |
| 124114 | pET21a-LysRS-His | Bacterial expression plasmid for PURE system components |
| 124115 | pET21a-MetRS-His | Bacterial expression plasmid for PURE system components |
| 124116 | pQE30-His-PheRS | Bacterial expression plasmid for PURE system components |
| 124117 | pET21a-ProRS-His | Bacterial expression plasmid for PURE system components |
| 124118 | pET21a-SerRS-His | Bacterial expression plasmid for PURE system components |
| 124119 | pQE30-His-ThrRS | Bacterial expression plasmid for PURE system components |
| 124120 | pET21a-TrpRS-His | Bacterial expression plasmid for PURE system components |
| 124121 | pET21a-TyrRS-His | Bacterial expression plasmid for PURE system components |
| 124122 | pET21a-ValRS-His | Bacterial expression plasmid for PURE system components |
| 124123 | pQE30-His-IF1 | Bacterial expression plasmid for PURE system components |
| 124124 | pQE30-His-IF2 | Bacterial expression plasmid for PURE system components |
| 124125 | pQE30-His-IF3 | Bacterial expression plasmid for PURE system components |
| 124126 | pQE60-EFG-His | Bacterial expression plasmid for PURE system components |
| 124127 | pQE60-EFTu-His | Bacterial expression plasmid for PURE system components |
| 124128 | pQE60-EFTs-His | Bacterial expression plasmid for PURE system components |
| 124130 | pET15b-His-RF2 | Bacterial expression plasmid for PURE system components |
| 124131 | pQE30-His-RF3 | Bacterial expression plasmid for PURE system components |
| 124132 | pQE60-RRF-His | Bacterial expression plasmid for PURE system components |
| 124133 | pET21a-MTF-His | Bacterial expression plasmid for PURE system components |
| 124134 | pQE30-His-CKM | Bacterial expression plasmid for PURE system components |
| 124135 | pET29b-AK1-His | Bacterial expression plasmid for PURE system components |
| 124136 | pQE30-His-NDK | Bacterial expression plasmid for PURE system components |
| 124137 | pET29b-IPP1-His | Bacterial expression plasmid for PURE system components |
| 124138 | pQE30-His-T7RNAP | Bacterial expression plasmid for PURE system components |