Gerhart Ryffel Lab Materials

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Title	Authors	Publication
A distant upstream promoter of the HNF-4alpha gene connects the transcription factors involved in maturity-onset diabetes of the young.	Thomas H, Jaschkowitz K, Bulman M, Frayling TM, Mitchell SM, Roosen S, Lingott-Frieg A, Tack CJ, Ellard S, Ryffel GU, Hattersley AT	Hum Mol Genet. 2001 Sep 15. 10(19):2089-97.
A systematic analysis of the 3'UTR of HNF4A mRNA reveals an interplay of regulatory elements including miRNA target sites.	Wirsing A, Senkel S, Klein-Hitpass L, Ryffel GU	PLoS One. 2011;6(11):e27438. Epub 2011 Nov 30.
Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1alpha promoter in transgenic Xenopus.	Ryffel GU, Lingott A	Mech Dev. 2000 Jan . 90(1):65-75.
Double conditional human embryonic kidney cell line based on FLP and PhiC31 mediated transgene integration.	Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU	BMC Res Notes. 2011 Oct 18;4:420.
FLP and Cre recombinase function in Xenopus embryos.	Werdien D, Peiler G, Ryffel GU	Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis.	Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, Senkel S, Ryffel GU, Waldner C	Transgenic Res. 2009 Aug . 18(4):595-605.
Heat-shock mediated overexpression of HNF1beta mutations has differential effects on gene expression in the Xenopus pronephric kidney.	Sauert K, Kahnert S, Roose M, Gull M, Brandli AW, Ryffel GU, Waldner C	PLoS One. 2012;7(3):e33522. Epub 2012 Mar 15.
HNF1B mutations associate with hypomagnesemia and renal magnesium wasting.	Adalat S, Woolf AS, Johnstone KA, Wirsing A, Harries LW, Long DA, Hennekam RC, Ledermann SE, Rees L, van't Hoff W, Marks SD, Trompeter RS, Tullus K, Winyard PJ, Cansick J, Mushtaq I, Dhillon HK, Bingham C, Edghill EL, Shroff R, Stanescu H, Ryffel GU, Ellard S, Bockenhauer D	J Am Soc Nephrol. 2009 May . 20(5):1123-31.
Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line.	Senkel S, Lucas B, Klein-Hitpass L, Ryffel GU	Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90.
Improved conditional expression systems resulting in physiological level of HNF4alpha expression confirm HNF4alpha induced apoptosis in the pancreatic beta-cell line INS-1.	Senkel S, Waldner C, Ryffel GU, Thomas H	BMC Res Notes. 2009 . 2():210.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.11.893545.30530	30530	pCSCMV:tdTomato	tdTomato	Mammalian Expression	Red fluorescent Xenopus laevis: a new tool for grafting analysis. BMC Dev Biol. 2009 . 9():37.
100.8.264412.31104	31104	FR_HNF1A	HNF1A (HNF1A Human)	Mammalian Expression	Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line. Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90.
100.3.009979.31100	31100	FR_HNF4A2	HNF4A (HNF4A Human)	Mammalian Expression	Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line. Nucleic Acids Res. 2004 . 32(19):e150.
50.90.635687.31128	31128	pCMV:DsRed(FRT)GFP	DsRed(FRT)GFP	Mammalian Expression	FLP and Cre recombinase function in Xenopus embryos. Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
50.18.481013.31132	31132	pCSCre	Cre recombinase (cre bacteriophage P1)	Mammalian Expression	FLP and Cre recombinase function in Xenopus embryos. Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
50.1.736228.31130	31130	pCSFLPe	FLP recombinase	Mammalian Expression	FLP and Cre recombinase function in Xenopus embryos. Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
50.1.019839.31110	31110	ACE2(-1119)-luc	ACE2 promoter (ACE2 Human)	Mammalian Expression, Luciferase	Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line. Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90.
50.0.538731.31101	31101	FR_HNF1B	HNF1B (HNF1B Human)	Mammalian Expression	Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line. Nucleic Acids Res. 2004 . 32(19):e150.
20.4.534556.31106	31106	OPN(-1206)-luc	osteopontin promoter (SPP1 Human)	Mammalian Expression, Luciferase	Identification of target genes of the transcription factor HNF1beta and HNF1alpha in a human embryonic kidney cell line. Biochim Biophys Acta. 2005 Dec 20. 1731(3):179-90.
20.2.486802.31099	31099	FR_HNF6	HNF6 (ONECUT1 Human)	Mammalian Expression	Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line. Nucleic Acids Res. 2004 . 32(19):e150.