Alessio Ciulli Lab Materials

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Title	Authors	Publication
Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase Complex.	Cardote TAF, Gadd MS, Ciulli A	Structure. 2017 Jun 6;25(6):901-911.e3. doi: 10.1016/j.str.2017.04.009.
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders.	Kroupova A, Spiteri VA, Rutter ZJ, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya AJ, Rodriguez-Rios M, Sturbaut M, Lynch DM, Farnaby W, Nakasone MA, Zollman D, Ciulli A	Nat Commun. 2024 Oct 15;15(1):8885. doi: 10.1038/s41467-024-52871-9.
Development of BromoTag: A "Bump-and-Hole"-PROTAC System to Induce Potent, Rapid, and Selective Degradation of Tagged Target Proteins.	Bond AG, Craigon C, Chan KH, Testa A, Karapetsas A, Fasimoye R, Macartney T, Blow JJ, Alessi DR, Ciulli A	J Med Chem. 2021 Oct 28;64(20):15477-15502. doi: 10.1021/acs.jmedchem.1c01532. Epub 2021 Oct 15.
Targeted protein degradation via intramolecular bivalent glues.	Hsia O, Hinterndorfer M, Cowan AD, Iso K, Ishida T, Sundaramoorthy R, Nakasone MA, Imrichova H, Schatz C, Rukavina A, Husnjak K, Wegner M, Correa-Saez A, Craigon C, Casement R, Maniaci C, Testa A, Kaulich M, Dikic I, Winter GE, Ciulli A	Nature. 2024 Mar;627(8002):204-211. doi: 10.1038/s41586-024-07089-6. Epub 2024 Feb 21.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
50.1240.905331.215330	215330	His6-TEV-CRBN-midi	CRBN (CRBN Human)	Bacterial Expression	Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders. Nat Commun. 2024 Oct 15;15(1):8885. doi: 10.1038/s41467-024-52871-9.
20.20.643758.204500	204500	pIVM_02	amino acids 54-213 VHL (VHL Human)	Bacterial Expression	Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase Complex. Structure. 2017 Jun 6;25(6):901-911.e3. doi: 10.1016/j.str.2017.04.009.
20.19.664328.204501	204501	pIVM_26	EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)	Bacterial Expression	Crystal Structure of the Cul2-Rbx1-EloBC-VHL Ubiquitin Ligase Complex. Structure. 2017 Jun 6;25(6):901-911.e3. doi: 10.1016/j.str.2017.04.009.
	231711	KHC064 pX335 BRD2 K1-1a	gRNA for Brd2 and Cas9D10A (BRD2 Human)	Mammalian Expression, CRISPR	Development of BromoTag: A "Bump-and-Hole"-PROTAC System to Induce Potent, Rapid, and Selective Degradation of Tagged Target Proteins. J Med Chem. 2021 Oct 28;64(20):15477-15502. doi: 10.1021/acs.jmedchem.1c01532. Epub 2021 Oct 15.
	231712	KHC065 pBABE pU6 BRD2 K1-2C	gRNA for Brd2 and Cas9D10A (BRD2 Human)	Mammalian Expression, CRISPR	Development of BromoTag: A "Bump-and-Hole"-PROTAC System to Induce Potent, Rapid, and Selective Degradation of Tagged Target Proteins. J Med Chem. 2021 Oct 28;64(20):15477-15502. doi: 10.1021/acs.jmedchem.1c01532. Epub 2021 Oct 15.
	231713	KHC068 BRD2-KI-GFP-2A-L387A	Brd2 (BRD2 Human)	Mammalian Expression, CRISPR	Development of BromoTag: A "Bump-and-Hole"-PROTAC System to Induce Potent, Rapid, and Selective Degradation of Tagged Target Proteins. J Med Chem. 2021 Oct 28;64(20):15477-15502. doi: 10.1021/acs.jmedchem.1c01532. Epub 2021 Oct 15.
	233886	10xHis-3C-BRD4(42-459) BD1-BD2	BRD4 (BRD4 Human)	Bacterial Expression	Targeted protein degradation via intramolecular bivalent glues. Nature. 2024 Mar;627(8002):204-211. doi: 10.1038/s41586-024-07089-6. Epub 2024 Feb 21.
	233887	His-GST-TEV-BRD4(42-459) BD1-BD2	BRD4 (BRD4 Human)	Bacterial Expression	Targeted protein degradation via intramolecular bivalent glues. Nature. 2024 Mar;627(8002):204-211. doi: 10.1038/s41586-024-07089-6. Epub 2024 Feb 21.