Robert Tjian Lab Materials

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Title	Authors	Publication
3D ATAC-PALM: super-resolution imaging of the accessible genome.	Xie L, Dong P, Chen X, Hsieh TS, Banala S, De Marzio M, English BP, Qi Y, Jung SK, Kieffer-Kwon KR, Legant WR, Hansen AS, Schulmann A, Casellas R, Zhang B, Betzig E, Lavis LD, Chang HY, Tjian R, Liu Z	Nat Methods. 2020 Apr;17(4):430-436. doi: 10.1038/s41592-020-0775-2. Epub 2020 Mar 16.
Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif.	Courey AJ, Tjian R	Cell. 1988 Dec 2. 55(5):887-98.
Analysis of the DNA-binding and activation properties of the human transcription factor AP-2.	Williams T, Tjian R	Genes Dev. 1991 Apr . 5(4):670-82.
Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation.	Ruppert S, Wang EH, Tjian R	Nature. 1993 Mar 11. 362(6416):175-9.
dCas9-targeted locus-specific protein isolation method identifies histone gene regulators.	Tsui C, Inouye C, Levy M, Lu A, Florens L, Washburn MP, Tjian R	Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):E2734-E2741. doi: 10.1073/pnas.1718844115. Epub 2018 Mar 5.
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.	Kadonaga JT, Carner KR, Masiarz FR, Tjian R	Cell. 1987 Dec 24. 51(6):1079-90.
Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection.	Graham TGW, Dugast-Darzacq C, Dailey GM, Nguyenla XH, Van Dis E, Esbin MN, Abidi A, Stanley SA, Darzacq X, Tjian R	PLoS One. 2021 Feb 3;16(2):e0246647. doi: 10.1371/journal.pone.0246647. eCollection 2021.
Requirement of tissue-selective TBP-associated factor TAFII105 in ovarian development.	Freiman RN, Albright SR, Zheng S, Sha WC, Hammer RE, Tjian R	Science. 2001 Sep 14. 293(5537):2084-7.
Sp1 plasmids	(unpublished)
Transcription factor AP-4 contains multiple dimerization domains that regulate dimer specificity.	Hu YF, Luscher B, Admon A, Mermod N, Tjian R	Genes Dev. 1990 Oct . 4(10):1741-52.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.1091.224242.166944	166944	pET-28a_6H-TAQ_E602D	Thermus aquaticus DNA polymerase	Bacterial Expression	Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection. PLoS One. 2021 Feb 3;16(2):e0246647. doi: 10.1371/journal.pone.0246647. eCollection 2021.
100.46.753716.12098	12098	RSV-Sp1	Sp1 (SP1 Human)	Mammalian Expression	Sp1 plasmids (unpublished)
100.3.223195.12097	12097	CMV-Sp1	Sp1 (SP1 Human)	Mammalian Expression	Sp1 plasmids (unpublished)
50.18.671530.166945	166945	pET-28a_6H-MMLV_RT_D524N-6H	gag-pol	Bacterial Expression	Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection. PLoS One. 2021 Feb 3;16(2):e0246647. doi: 10.1371/journal.pone.0246647. eCollection 2021.
50.15.233611.139746	139746	pLX_lenti_osTir1_9Myc_P2A_Bsd	rice E3 ligase Tir1 (osTir1)	Mammalian Expression	3D ATAC-PALM: super-resolution imaging of the accessible genome. Nat Methods. 2020 Apr;17(4):430-436. doi: 10.1038/s41592-020-0775-2. Epub 2020 Mar 16.
50.6.720434.111140	111140	pCT310	dCas9	Bacterial Expression	dCas9-targeted locus-specific protein isolation method identifies histone gene regulators. Proc Natl Acad Sci U S A. 2018 Mar 20;115(12):E2734-E2741. doi: 10.1073/pnas.1718844115. Epub 2018 Mar 5.
50.2.116726.17997	17997	CMV-HA-hTAF1	hTAF1 (TAF1 Human)	Mammalian Expression	Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation. Nature. 1993 Mar 11. 362(6416):175-9.
50.1.056012.12100	12100	SP (RSV) AP-2	AP-2 (TFAP2A Human)	Mammalian Expression	Analysis of the DNA-binding and activation properties of the human transcription factor AP-2. Genes Dev. 1991 Apr . 5(4):670-82.
50.0.364408.12096	12096	pBS Sp1 full length	Sp1 (SP1 Human)		Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. Cell. 1987 Dec 24. 51(6):1079-90.
20.0.481603.12095	12095	pPac Sp1	Sp1 (SP1 Human)	Insect Expression	Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif. Cell. 1988 Dec 2. 55(5):887-98.