Jesse Zalatan Lab Materials

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Title	Authors	Publication
CRISPR-Cas tools for simultaneous transcription & translation control in bacteria.	Cardiff RAL, Faulkner ID, Beall JG, Carothers JM, Zalatan JG	Nucleic Acids Res. 2024 May 22;52(9):5406-5419. doi: 10.1093/nar/gkae275.
CRISPR-Cas-Mediated Chemical Control of Transcriptional Dynamics in Yeast.	Cunningham-Bryant D, Sun J, Fernandez B, Zalatan JG	Chembiochem. 2019 Jun 14;20(12):1519-1523. doi: 10.1002/cbic.201800823. Epub 2019 Apr 26.
Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements.	Fontana J, Dong C, Kiattisewee C, Chavali VP, Tickman BI, Carothers JM, Zalatan JG	Nat Commun. 2020 Apr 1;11(1):1618. doi: 10.1038/s41467-020-15454-y.
Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants.	Kiattisewee C, Karanjia AV, Legut M, Daniloski Z, Koplik SE, Nelson J, Kleinstiver BP, Sanjana NE, Carothers JM, Zalatan JG	ACS Synth Biol. 2022 Nov 15. doi: 10.1021/acssynbio.2c00405.
Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida.	Kiattisewee C, Dong C, Fontana J, Sugianto W, Peralta-Yahya P, Carothers JM, Zalatan JG	Metab Eng. 2021 Apr 27. pii: S1096-7176(21)00063-X. doi: 10.1016/j.ymben.2021.04.002.
Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria.	Dong C, Fontana J, Patel A, Carothers JM, Zalatan JG	Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
The Axin scaffold protects the kinase GSK3beta from cross-pathway inhibition.	Gavagan M, Jameson N, Zalatan JG	Elife. 2023 Aug 7;12:e85444. doi: 10.7554/eLife.85444.
The Scaffold Protein Axin Promotes Signaling Specificity within the Wnt Pathway by Suppressing Competing Kinase Reactions.	Gavagan M, Fagnan E, Speltz EB, Zalatan JG	Cell Syst. 2020 Jun 24;10(6):515-525.e5. doi: 10.1016/j.cels.2020.05.002. Epub 2020 Jun 17.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
	113314	pCD005	W108 scRNA_MS2	Bacterial Expression, CRISPR	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
	113315	pCD061	W108 scRNA.b1_MS2	Bacterial Expression, CRISPR	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
	113316	pCD175	dCas9, MCP-T4.AsiA, rpoD_F563Y	Bacterial Expression, CRISPR	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
	113317	pCD185	dCas9 and MCP-SoxS_R93A	Bacterial Expression, CRISPR	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
	113318	pCD227	dCas9 and MCP-SoxS_R93A	Bacterial Expression, CRISPR	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
	113319	pCD294.9	dCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7	Bacterial Expression, CRISPR	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
	113320	pCD296.7	dCas9, MCP-TetD, J107 scRNA.b1_MS2	Bacterial Expression, CRISPR	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
	113321	pCD315	scRNA.b1_MS2	Bacterial Expression, CRISPR	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
	113322	pJF076Sa	mRFP1	Bacterial Expression	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.
	113323	pJF093	dCas9 and MCP-SoxS_R93A	Bacterial Expression, CRISPR	Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria. Nat Commun. 2018 Jun 27;9(1):2489. doi: 10.1038/s41467-018-04901-6.