Zhili Rong Lab Materials

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Title	Authors	Publication
Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system.	Huang H, Zhang X, Lv J, Yang H, Wang X, Ma S, Shao R, Peng X, Lin Y, Rong Z	Protein Cell. 2020 Apr;11(4):299-303. doi: 10.1007/s13238-020-00690-1.
Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity.	Huang H, Huang G, Tan Z, Hu Y, Shan L, Zhou J, Zhang X, Ma S, Lv W, Huang T, Liu Y, Wang D, Zhao X, Lin Y, Rong Z	BMC Biol. 2022 Apr 25;20(1):91. doi: 10.1186/s12915-022-01296-1.
Enhancing site-specific DNA integration by a Cas9 nuclease fused with a DNA donor-binding domain.	Ma S, Wang X, Hu Y, Lv J, Liu C, Liao K, Guo X, Wang D, Lin Y, Rong Z	Nucleic Acids Res. 2020 Oct 9;48(18):10590-10601. doi: 10.1093/nar/gkaa779.
iKA-CRISPR hESCs for inducible and multiplex orthogonal gene knockout and activation.	Ma S, Lv J, Sun J, Tang P, Li H, Zhou H, Zhang Z, Lin Y, Rong Z	FEBS Lett. 2018 Jun 5. doi: 10.1002/1873-3468.13127.
MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo.	Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, Zhao S, Feng J, Huang G, Wan QL, Liu J, Huang H, Luan B, Wang D, Zhao X, Lin Y, Zhou Q, Zhang ZN, Rong Z	Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
20.1.221102.169910	169910	CMV-VPR-S-L1-dCjCas9 MiniCAFE	MiniCAFE	Mammalian Expression, CRISPR	MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174.
	112261	Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1	spCas9-p300 (EP300 Human, Synthetic)	Mammalian Expression	iKA-CRISPR hESCs for inducible and multiplex orthogonal gene knockout and activation. FEBS Lett. 2018 Jun 5. doi: 10.1002/1873-3468.13127.
	112262	pU6-EF-gAAVS1	g-AAVS1	Mammalian Expression	iKA-CRISPR hESCs for inducible and multiplex orthogonal gene knockout and activation. FEBS Lett. 2018 Jun 5. doi: 10.1002/1873-3468.13127.
	169912	pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D	dCjCas9	Mammalian Expression, CRISPR	MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174.
	169913	pRGEN-CMV-VPR-L1-CjCas9 V-D	dCjCas9	Mammalian Expression, CRISPR	MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174.
	169914	pRGEN-CMV-VPR-WT CjCas9 V-WT	CjCas9	Mammalian Expression, CRISPR	MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174.
	169915	pU6-cj-E sgRNA		Mammalian Expression, CRISPR	MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174.
	169916	pHR_PGK_antiCD19_Z3_Gal4VP64	antiCD19-Notch3-GL4VP64	Mammalian Expression, Lentiviral	Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system. Protein Cell. 2020 Apr;11(4):299-303. doi: 10.1007/s13238-020-00690-1.
	169917	pHR_PGK_antiCD19_eZ3_Gal4VP64	antiCD19-Notch3-GL4VP64	Mammalian Expression, Lentiviral	Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system. Protein Cell. 2020 Apr;11(4):299-303. doi: 10.1007/s13238-020-00690-1.
	169918	pHR_PGK_antiCD19_H1_Gal4VP64	antiCD19-Notch1-GL4VP64	Mammalian Expression, Lentiviral	Cell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system. Protein Cell. 2020 Apr;11(4):299-303. doi: 10.1007/s13238-020-00690-1.