Maarten Merkx Lab Materials

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Title	Authors	Publication
Bioluminescent Antibodies through Photoconjugation of Protein G-Luciferase Fusion Proteins.	Wouters SFA, Vugs WJP, Arts R, de Leeuw NM, Teeuwen RWH, Merkx M	Bioconjug Chem. 2020 Mar 18;31(3):656-662. doi: 10.1021/acs.bioconjchem.9b00804. Epub 2020 Jan 22.
Bright Bioluminescent BRET Sensor Proteins for Measuring Intracellular Caspase Activity.	den Hamer A, Dierickx P, Arts R, de Vries JSPM, Brunsveld L, Merkx M	ACS Sens. 2017 Jun 23;2(6):729-734. doi: 10.1021/acssensors.7b00239. Epub 2017 May 31.
Colorful Protein-Based Fluorescent Probes for Collagen Imaging.	Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M	PLoS One. 2014 Dec 9;9(12):e114983. doi: 10.1371/journal.pone.0114983. eCollection 2014.
Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc.	Aper SJ, Dierickx P, Merkx M	ACS Chem Biol. 2016 Oct 21;11(10):2854-2864. Epub 2016 Sep 1.
eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging.	Hessels AM, Chabosseau P, Bakker MH, Engelen W, Rutter GA, Taylor KM, Merkx M	ACS Chem Biol. 2015 Sep 18;10(9):2126-34. doi: 10.1021/acschembio.5b00211. Epub 2015 Jul 20.
Genetically encoded FRET sensors to monitor intracellular Zn(2+) homeostasis.	Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M	Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
Glow-in-the-Dark Infectious Disease Diagnostics Using CRISPR-Cas9-Based Split Luciferase Complementation.	van der Veer HJ, van Aalen EA, Michielsen CMS, Hanckmann ETL, Deckers J, van Borren MMGJ, Flipse J, Loonen AJM, Schoeber JPH, Merkx M	ACS Cent Sci. 2023 Mar 15;9(4):657-667. doi: 10.1021/acscentsci.2c01467. eCollection 2023 Apr 26.
MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor.	Lindenburg LH, Vinkenborg JL, Oortwijn J, Aper SJ, Merkx M	PLoS One. 2013 Dec 2;8(12):e82009. doi: 10.1371/journal.pone.0082009.
Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers.	Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M	Biochemistry. 2006 Nov 7. 45(44):13183-92.
Ratiometric Bioluminescent Zinc Sensor Proteins to Quantify Serum and Intracellular Free Zn(2).	Michielsen CMS, van Aalen EA, Merkx M	ACS Chem Biol. 2022 Jun 17;17(6):1567-1576. doi: 10.1021/acschembio.2c00227. Epub 2022 May 25.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.9.731693.61603	61603	pET28a-EGFP-CNA35	EGFP and CNA35	Bacterial Expression	Colorful Protein-Based Fluorescent Probes for Collagen Imaging. PLoS One. 2014 Dec 9;9(12):e114983. doi: 10.1371/journal.pone.0114983. eCollection 2014.
50.34.151267.61607	61607	pET28a-mCherry-CNA35	mCherry and CNA35	Bacterial Expression	Colorful Protein-Based Fluorescent Probes for Collagen Imaging. PLoS One. 2014 Dec 9;9(12):e114983. doi: 10.1371/journal.pone.0114983. eCollection 2014.
50.3.140229.50742	50742	pCMVMagFRET-1	gene encoding for MagFRET-1	Mammalian Expression	MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. 2013 Dec 2;8(12):e82009. doi: 10.1371/journal.pone.0082009.
50.2.617355.22236	22236	peCALWY-4	eCALWY-4 Zn(II) sensor (ATOX1 GFP's, Human)	Mammalian Expression	Genetically encoded FRET sensors to monitor intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
20.10.134610.98287	98287	mNeonGreen-DEVD-NanoLuc	mNeonGreen-LEHD-NanoLuc	Mammalian Expression	Bright Bioluminescent BRET Sensor Proteins for Measuring Intracellular Caspase Activity. ACS Sens. 2017 Jun 23;2(6):729-734. doi: 10.1021/acssensors.7b00239. Epub 2017 May 31.
20.3.790274.61606	61606	pET28a-tdTomato-CNA35	tdTomato and CNA35	Bacterial Expression	Colorful Protein-Based Fluorescent Probes for Collagen Imaging. PLoS One. 2014 Dec 9;9(12):e114983. doi: 10.1371/journal.pone.0114983. eCollection 2014.
20.3.139106.21760	21760	pET28CLY1	ECFP -EYFP linked by flexible one SGGSGG repeats	Bacterial Expression	Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers. Biochemistry. 2006 Nov 7. 45(44):13183-92.
20.2.044366.61602	61602	pET28a-mTurquoise2-CNA35	mTurqouise and CNA35	Bacterial Expression	Colorful Protein-Based Fluorescent Probes for Collagen Imaging. PLoS One. 2014 Dec 9;9(12):e114983. doi: 10.1371/journal.pone.0114983. eCollection 2014.
20.1.374968.50756	50756	pCMVMagFRET-6	gene encoding for MagFRET-6	Mammalian Expression	MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. 2013 Dec 2;8(12):e82009. doi: 10.1371/journal.pone.0082009.
20.0.940406.21769	21769	pET28CLY9	ECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats	Bacterial Expression	Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers. Biochemistry. 2006 Nov 7. 45(44):13183-92.