Bon-Kyoung Koo Lab Materials

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Title	Authors	Publication
One-step generation of conditional and reversible gene knockouts.	Andersson-Rolf A, Mustata RC, Merenda A, Kim J, Perera S, Grego T, Andrews K, Tremble K, Silva JC, Fink J, Skarnes WC, Koo BK	Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids.	Andersson-Rolf A, Merenda A, Mustata RC, Li T, Dietmann S, Koo BK	Dev Biol. 2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 10.1016/j.ydbio.2016.10.016.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
50.1.349136.84539	84539	pUC118-FLIP-FlpE-Puro	FLIP-FlpE - Puro	Mouse Targeting, Cre/Lox	One-step generation of conditional and reversible gene knockouts. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
50.0.595381.84538	84538	pUC118-FLIP-Puro	FLIP - Puro	Mouse Targeting, Cre/Lox	One-step generation of conditional and reversible gene knockouts. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
20.0.420999.84881	84881	4 gRNA concatemer		Mammalian Expression, Retroviral	Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 10.1016/j.ydbio.2016.10.016.
	84540	pUC118-mCtnnb1-FLIP-Puro	mouse beta-catenin FLIP - Puro targeting vector	Mouse Targeting, Cre/Lox	One-step generation of conditional and reversible gene knockouts. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
	84541	pUC118-mCtnnb1-FLIP-FlpE-Puro	mouse beta-catenin FLIP-FlpE - Puro targeting vector	Mouse Targeting, Cre/Lox	One-step generation of conditional and reversible gene knockouts. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
	84542	gRNA-mCtnnb1	mCtnnb1 gRNA	CRISPR	One-step generation of conditional and reversible gene knockouts. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156.
	84879	2 gRNA concatmer		Mammalian Expression, Retroviral	Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 10.1016/j.ydbio.2016.10.016.
	84880	3 gRNA concatemer		Mammalian Expression, Retroviral	Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids. Dev Biol. 2016 Oct 27. pii: S0012-1606(16)30479-1. doi: 10.1016/j.ydbio.2016.10.016.