Brian Pfleger Lab Materials

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Title	Authors	Publication
A transcription activator-like effector (TALE) induction system mediated by proteolysis.	Copeland MF, Politz MC, Johnson CB, Markley AL, Pfleger BF	Nat Chem Biol. 2016 Apr;12(4):254-60. doi: 10.1038/nchembio.2021. Epub 2016 Feb 8.
Anaerobic production of medium-chain fatty alcohols via a beta-reduction pathway.	Mehrer CR, Incha MR, Politz MC, Pfleger BF	Metab Eng. 2018 Jul;48:63-71. doi: 10.1016/j.ymben.2018.05.011. Epub 2018 May 25.
Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002.	Zess EK, Begemann MB, Pfleger BF	Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. Epub 2015 Sep 3.
CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002.	Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger BF	Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 10.1016/j.ymben.2016.07.007.
Expanding the synthetic biology toolbox of Cupriavidus necator for establishing fatty acid production.	Mishra S, Perkovich PM, Mitchell WP, Venkataraman M, Pfleger B	J Ind Microbiol Biotechnol. 2024 Feb 16:kuae008. doi: 10.1093/jimb/kuae008.
Genetic tools for reliable gene expression and recombineering in Pseudomonas putida.	Cook TB, Rand JM, Nurani W, Courtney DK, Liu SA, Pfleger BF	J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3).	Jones CM, Korosh TC, Nielsen DR, Pfleger BF	Appl Microbiol Biotechnol. 2021 Feb;105(3):1147-1158. doi: 10.1007/s00253-020-11085-x. Epub 2021 Jan 14.
Stepwise genetic engineering of Pseudomonas putida enables robust heterologous production of prodigiosin and glidobactin A.	Cook TB, Jacobson TB, Venkataraman MV, Hofstetter H, Amador-Noguez D, Thomas MG, Pfleger BF	Metab Eng. 2021 Jun 24;67:112-124. doi: 10.1016/j.ymben.2021.06.004.
Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002.	Markley AL, Begemann MB, Clarke RE, Gordon GC, Pfleger BF	ACS Synth Biol. 2015 May 15;4(5):595-603. doi: 10.1021/sb500260k. Epub 2014 Sep 25.
Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.	Venkataraman M, Ynigez-Gutierrez A, Infante V, MacIntyre A, Fernandes-Junior PI, Ane JM, Pfleger B	ACS Synth Biol. 2023 Dec 15;12(12):3623-3634. doi: 10.1021/acssynbio.3c00414. Epub 2023 Nov 21.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
20.196.057933.106395	106395	pBb(RSF1010)1k-GFPuv	GFPuv	Bacterial Expression	Genetic tools for reliable gene expression and recombineering in Pseudomonas putida. J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
20.195.142824.106394	106394	pBb(RK2)1k-GFPuv	GFPuv	Bacterial Expression	Genetic tools for reliable gene expression and recombineering in Pseudomonas putida. J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
20.25.345449.106382	106382	pBBR1k-GFPuv	GFPuv	Bacterial Expression	Genetic tools for reliable gene expression and recombineering in Pseudomonas putida. J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
20.2.080568.106397	106397	pgRNAtet-lvaA	sgRNA towards lvaA in Pseudomonas putida	Bacterial Expression, CRISPR, Synthetic Biology	Genetic tools for reliable gene expression and recombineering in Pseudomonas putida. J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
20.1.903535.110141	110141	pRK2-AraE	araE	Bacterial Expression	Genetic tools for reliable gene expression and recombineering in Pseudomonas putida. J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
20.1.608856.110142	110142	pJOE_pvdJ		Bacterial Expression	Genetic tools for reliable gene expression and recombineering in Pseudomonas putida. J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
20.0.961374.106386	106386	pBBR1k-con-GFPuv	GFPuv	Bacterial Expression	Genetic tools for reliable gene expression and recombineering in Pseudomonas putida. J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
	81124	pEZ01	GFP		Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002. Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. Epub 2015 Sep 3.
	81125	pEZ02	GFP		Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002. Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. Epub 2015 Sep 3.
	81127	pEZ04	GFP		Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002. Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. Epub 2015 Sep 3.