Wesley Sundquist Lab Materials

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Title	Authors	Publication
ALIX-CHMP4 interactions in the human ESCRT pathway.	McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP	Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 10.1073/pnas.0801567105. Epub 2008 May 29.
Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission.	Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, Sundquist WI	Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779.
Designed proteins induce the formation of nanocage-containing extracellular vesicles.	Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King NP, Sundquist WI	Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30.
Identification of abscission checkpoint bodies as structures that regulate ESCRT factors to control abscission timing.	Williams LK, Mackay DR, Whitney MA, Couldwell GC, Sundquist WI, Ullman KS	Elife. 2021 Aug 4;10. pii: 63743. doi: 10.7554/eLife.63743.
Interactions between AMOT PPxY Motifs and NEDD4L WW Domains Function in HIV-1 Release.	Rheinemann L, Thompson T, Mercenne G, Paine EL, Peterson FC, Volkman BF, Alam SL, Alian A, Sundquist WI	J Biol Chem. 2021 Jul 17:100975. doi: 10.1016/j.jbc.2021.100975.
LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells.	Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A	Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 10.1073/pnas.1613916114. Epub 2017 Feb 27.
Membrane constriction and thinning by sequential ESCRT-III polymerization.	Nguyen HC, Talledge N, McCullough J, Sharma A, Moss FR 3rd, Iwasa JH, Vershinin MD, Sundquist WI, Frost A	Nat Struct Mol Biol. 2020 Apr;27(4):392-399. doi: 10.1038/s41594-020-0404-x. Epub 2020 Apr 6.
Plasmids for creating inducible cell lines expressing ALIX-GFP	(unpublished)
Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids.	Li YL, Chandrasekaran V, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK, Jensen GJ, Sundquist WI	Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
Reconstitution and visualization of HIV-1 capsid-dependent replication and integration in vitro.	Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, Sundquist WI	Science. 2020 Oct 9;370(6513). pii: 370/6513/eabc8420. doi: 10.1126/science.abc8420.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.14.355503.80351	80351	pEGFP-VSP4-E228Q	VPS4-E228Q (VPS4A Human)	Mammalian Expression	Designed proteins induce the formation of nanocage-containing extracellular vesicles. Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30.
50.48.117821.174089	174089	pLVX-SRRM2-mCherry	SRRM2 (SRRM2 Human)	Mammalian Expression, Lentiviral	Identification of abscission checkpoint bodies as structures that regulate ESCRT factors to control abscission timing. Elife. 2021 Aug 4;10. pii: 63743. doi: 10.7554/eLife.63743.
50.23.478456.83375	83375	pCMV-Myc-GFP	Myc-GFP	Mammalian Expression	Designed proteins induce the formation of nanocage-containing extracellular vesicles. Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30.
50.6.122151.89859	89859	pCINeoFlag ALIX	ALIX (PDCD6IP Human)	Mammalian Expression	Structure of the Bro1 domain protein BROX and functional analyses of the ALIX Bro1 domain in HIV-1 budding. PLoS One. 2011;6(12):e27466. doi: 10.1371/journal.pone.0027466. Epub 2011 Dec 5.
20.128.761648.79039	79039	pET3a-OSF-CypA	OneSTrEP-FLAG- Cyclophilin A (PPIA Human)	Bacterial Expression	Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
20.25.481771.97006	97006	pMGF182	CHMP7 (CHMP7 Human)	Mammalian Expression	LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 10.1073/pnas.1613916114. Epub 2017 Feb 27.
20.2.374504.83374	83374	pCMV-Myc-GFP-Vpr-RR	Myc-GFP-Vpr	Mammalian Expression	Designed proteins induce the formation of nanocage-containing extracellular vesicles. Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30.
20.1.405118.97005	97005	pMGF196	LEMD2 (LEMD2 Human)	Mammalian Expression	LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 10.1073/pnas.1613916114. Epub 2017 Feb 27.
	79029	pDONR221-OSF-TRIM5-21R(1-300)	TRIM5-21R (1-300)	Insect Expression	Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.
	79030	pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)	OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)	Insect Expression	Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Elife. 2016 Jun 2;5. pii: e16269. doi: 10.7554/eLife.16269.