Michael Jewett Lab Materials

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• Plasmids
• Bacterial Strains
• Articles

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Title	Authors	Publication
BioBits Bright: A fluorescent synthetic biology education kit.	Stark JC, Huang A, Nguyen PQ, Dubner RS, Hsu KJ, Ferrante TC, Anderson M, Kanapskyte A, Mucha Q, Packett JS, Patel P, Patel R, Qaq D, Zondor T, Burke J, Martinez T, Miller-Berry A, Puppala A, Reichert K, Schmid M, Brand L, Hill LR, Chellaswamy JF, Faheem N, Fetherling S, Gong E, Gonzalzles EM, Granito T, Koritsaris J, Nguyen B, Ottman S, Palffy C, Patel A, Skweres S, Slaton A, Woods T, Donghia N, Pardee K, Collins JJ, Jewett MC	Sci Adv. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107. eCollection 2018 Aug.
BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts.	Stark JC, Huang A, Hsu KJ, Dubner RS, Forbrook J, Marshalla S, Rodriguez F, Washington M, Rybnicky GA, Nguyen PQ, Hasselbacher B, Jabri R, Kamran R, Koralewski V, Wightkin W, Martinez T, Jewett MC	ACS Synth Biol. 2019 May 7. doi: 10.1021/acssynbio.8b00381.
Cell-free expression and characterization of multivalent rhamnose-binding lectins using biolayer interferometry.	Warfel KF, Laigre E, Sobol SE, Gillon E, Varrot A, Renaudet O, Dejeu J, Jewett MC, Imberty A	Glycobiology. 2023 Mar 7:cwad018. doi: 10.1093/glycob/cwad018.
Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation.	Hong SH, Ntai I, Haimovich AD, Kelleher NL, Isaacs FJ, Jewett MC	ACS Synth Biol. 2014 Jun 20;3(6):398-409. doi: 10.1021/sb400140t. Epub 2014 Jan 2.
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids.	Martin RW, Des Soye BJ, Kwon YC, Kay J, Davis RG, Thomas PM, Majewska NI, Chen CX, Marcum RD, Weiss MG, Stoddart AE, Amiram M, Ranji Charna AK, Patel JR, Isaacs FJ, Kelleher NL, Hong SH, Jewett MC	Nat Commun. 2018 Mar 23;9(1):1203. doi: 10.1038/s41467-018-03469-5.
Cell-Free Translation Quantification via a Fluorescent Minihelix.	Willi JA, Karim AS, Jewett MC	ACS Synth Biol. 2024 Jul 19;13(7):2253-2259. doi: 10.1021/acssynbio.4c00266. Epub 2024 Jul 9.
GlycoCAP: A Cell-Free, Bacterial Glycosylation Platform for Building Clickable Azido-Sialoglycoproteins.	Thames AH, Moons SJ, Wong DA, Boltje TJ, Bochner BS, Jewett MC	ACS Synth Biol. 2023 Apr 11. doi: 10.1021/acssynbio.3c00017.
Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles.	Hershewe JM, Warfel KF, Iyer SM, Peruzzi JA, Sullivan CJ, Roth EW, DeLisa MP, Kamat NP, Jewett MC	Nat Commun. 2021 Apr 22;12(1):2363. doi: 10.1038/s41467-021-22329-3.
Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1.	Hong SH, Kwon YC, Martin RW, Des Soye BJ, de Paz AM, Swonger KN, Ntai I, Kelleher NL, Jewett MC	Chembiochem. 2015 Mar 23;16(5):844-53. doi: 10.1002/cbic.201402708. Epub 2015 Mar 3.
Improving genomically recoded Escherichia coli to produce proteins containing non-canonical amino acids.	Perez JG, Carlson ED, Weisser O, Kofman C, Seki K, Des Soye BJ, Karim AS, Jewett MC	Biotechnol J. 2021 Dec 11:e2100330. doi: 10.1002/biot.202100330.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
50.45.381807.102634	102634	pJL1-sfGFP	sfGFP	Bacterial Expression	BioBits Bright: A fluorescent synthetic biology education kit. Sci Adv. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107. eCollection 2018 Aug.
50.23.624254.69496	69496	pJL1	Super folder GFP	Bacterial Expression	Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids. Nat Commun. 2018 Mar 23;9(1):1203. doi: 10.1038/s41467-018-03469-5.
20.1.905450.102630	102630	pJL1-mRFP1	mRFP1	Bacterial Expression	BioBits Bright: A fluorescent synthetic biology education kit. Sci Adv. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107. eCollection 2018 Aug.
	69342	SQ171/pRibo-T	Ribo-T	Bacterial Expression	Protein synthesis by ribosomes with tethered subunits. Nature. 2015 Jul 29. doi: 10.1038/nature14862.
	69343	SQ171fg/pRibo-T	Ribo-T	Bacterial Expression	Protein synthesis by ribosomes with tethered subunits. Nature. 2015 Jul 29. doi: 10.1038/nature14862.
	69344	SQ171fg/pCSacB	pCSacB		Protein synthesis by ribosomes with tethered subunits. Nature. 2015 Jul 29. doi: 10.1038/nature14862.
	69345	pLpp5oGFP	super folder GFP	Bacterial Expression	Protein synthesis by ribosomes with tethered subunits. Nature. 2015 Jul 29. doi: 10.1038/nature14862.
	69346	pT7oGFP	Super folder GFP	Unspecified	Protein synthesis by ribosomes with tethered subunits. Nature. 2015 Jul 29. doi: 10.1038/nature14862.
	69347	poRibo-T2	oRibo-T	Bacterial Expression	Protein synthesis by ribosomes with tethered subunits. Nature. 2015 Jul 29. doi: 10.1038/nature14862.
	102629	pJL1-mCherry	mCherry	Bacterial Expression	BioBits Bright: A fluorescent synthetic biology education kit. Sci Adv. 2018 Aug 1;4(8):eaat5107. doi: 10.1126/sciadv.aat5107. eCollection 2018 Aug.

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	ID	Bacterial Strain
20.10.948482.182778	182778	C321.∆A T7RNAP ompT- rne- lon-
	69493	MCJ.559
	69494	rEc.13
	69495	rEc.13.delA
	132780	CLM24 ∆lpxM
	182773	C321.∆A ompT- rne- lon-
	182774	C321.∆A ompT- rne- endA-
	182775	C321.∆A ompT- rne- endA- lon-
	182776	C321.∆A T7RNAP
	182777	C321.∆A T7RNAP ompT- rne-