Michael Nonet Lab Materials

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Title	Authors	Publication
Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange.	Nonet ML	Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
Landscape of target:guide homology effects on Cas9-mediated cleavage.	Fu BX, Hansen LL, Artiles KL, Nonet ML, Fire AZ	Nucleic Acids Res. 2014 Dec 16;42(22):13778-87. doi: 10.1093/nar/gku1102. Epub 2014 Nov 15.
Rapid generation of C. elegans single-copy transgenes combining RMCE and drug selection.	Nonet ML	Genetics. 2023 Apr 20:iyad072. doi: 10.1093/genetics/iyad072.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.7.671638.67881	67881	pHO4d-Cas9	Cas9	Bacterial Expression, CRISPR	Landscape of target:guide homology effects on Cas9-mediated cleavage. Nucleic Acids Res. 2014 Dec 16;42(22):13778-87. doi: 10.1093/nar/gku1102. Epub 2014 Nov 15.
20.2.416987.153083	153083	pLF3FShC		Worm Expression	Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
	153086	pNM3732	mec-4p GFP-C1 tbb-2 3'	Worm Expression	Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
	154108	pNM3749	LoxP-SEC-LoxP, FRT::rpl-28p::GFP::his-58::FRT3	Worm Expression, Cre/Lox	Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
	154109	pNM3746	LoxP-SEC-LoxP, FRT::rpl-28p::GFP::His-58::FRT3, mex-5p::FLP D5::sl2::mNG::glh2 3'	Worm Expression, Cre/Lox	Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
	154110	pNM3649	loxP::SEC::loxP::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3		Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
	154111	pNM3689	loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3		Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
	154112	pNM3724	tetO 7X::∆pes-10::GFP-C1::tbb-2 3'	Worm Expression	Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
	154113	pNM3725	UAS 11X::∆pes-10::GFP-C1::tbb-2 3'	Worm Expression	Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
	154114	pNM3726	QUAS 5X::∆pes-10::GFP-C1::tbb-2 3'	Worm Expression	Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange. Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.