John Spudich Lab Materials

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Title	Authors	Publication
Bacteriorhodopsin-like channelrhodopsins: Alternative mechanism for control of cation conductance.	Sineshchekov OA, Govorunova EG, Li H, Spudich JL	Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):E9512-E9519. doi: 10.1073/pnas.1710702114. Epub 2017 Oct 25.
Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis.	Govorunova EG, Sineshchekov OA, Li H, Janz R, Spudich JL	J Biol Chem. 2013 Oct 11;288(41):29911-22. doi: 10.1074/jbc.M113.505495. Epub 2013 Aug 30.
Conductance Mechanisms of Rapidly Desensitizing Cation Channelrhodopsins from Cryptophyte Algae.	Sineshchekov OA, Govorunova EG, Li H, Wang Y, Melkonian M, Wong GK, Brown LS, Spudich JL	mBio. 2020 Apr 21;11(2). pii: mBio.00657-20. doi: 10.1128/mBio.00657-20.
Extending the Time Domain of Neuronal Silencing with Cryptophyte Anion Channelrhodopsins.	Govorunova EG, Sineshchekov OA, Hemmati R, Janz R, Morelle O, Melkonian M, Wong GK, Spudich JL	eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-0174-18. doi: 10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun.
Kalium channelrhodopsins are natural light-gated potassium channels that mediate optogenetic inhibition.	Govorunova EG, Gou Y, Sineshchekov OA, Li H, Lu X, Wang Y, Brown LS, St-Pierre F, Xue M, Spudich JL	Nat Neurosci. 2022 Jun 20. pii: 10.1038/s41593-022-01094-6. doi: 10.1038/s41593-022-01094-6.
Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q	(unpublished)
Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics.	Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL	Science. 2015 Jun 25. pii: aaa7484.
New channelrhodopsin with a red-shifted spectrum and rapid kinetics from Mesostigma viride.	Govorunova EG, Spudich EN, Lane CE, Sineshchekov OA, Spudich JL	mBio. 2011 Jun 21;2(3):e00115-11. doi: 10.1128/mBio.00115-11. Print 2011.
RubyACRs, nonalgal anion channelrhodopsins with highly red-shifted absorption.	Govorunova EG, Sineshchekov OA, Li H, Wang Y, Brown LS, Spudich JL	Proc Natl Acad Sci U S A. 2020 Sep 15;117(37):22833-22840. doi: 10.1073/pnas.2005981117. Epub 2020 Sep 1.
Structural Foundations of Potassium Selectivity in Channelrhodopsins.	Govorunova EG, Sineshchekov OA, Brown LS, Bondar AN, Spudich JL	mBio. 2022 Dec 20;13(6):e0303922. doi: 10.1128/mbio.03039-22. Epub 2022 Nov 22.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
50.1.716130.67795	67795	pFUGW-hGtACR1-EYFP	hGtACR1	Mammalian Expression, Lentiviral	Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics. Science. 2015 Jun 25. pii: aaa7484.
50.1.443939.67877	67877	pFUGW-hGtACR2-EYFP	hGtACR2	Mammalian Expression, Lentiviral	Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics. Science. 2015 Jun 25. pii: aaa7484.
20.1.458193.177336	177336	HcKCR1_mCherry_pcDNA3.1	HcKCR1	Mammalian Expression	Kalium channelrhodopsins are natural light-gated potassium channels that mediate optogenetic inhibition. Nat Neurosci. 2022 Jun 20. pii: 10.1038/s41593-022-01094-6. doi: 10.1038/s41593-022-01094-6.
20.0.547636.69057	69057	pcDNA3.1_PsChR2_EYFP	PsChR2	Mammalian Expression	Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis. J Biol Chem. 2013 Oct 11;288(41):29911-22. doi: 10.1074/jbc.M113.505495. Epub 2013 Aug 30.
	88844	pFUGW-ZipACR-EYFP	ZipACR-NotI-EYFP	Lentiviral	The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity. Sci Rep. 2017 Mar 3;7:43358. doi: 10.1038/srep43358.
	103137	pcDNA3.1-GcACR_457-EYFP	Anion channelrhodopsin GcACR_457	Mammalian Expression	The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity. Sci Rep. 2017 Mar 3;7:43358. doi: 10.1038/srep43358.
	103138	pcDNA3.1-GcACR_145-EYFP	Anion channelrhodopsin GcACR_145	Mammalian Expression	The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity. Sci Rep. 2017 Mar 3;7:43358. doi: 10.1038/srep43358.
	103142	pcDNA3.1-GcACR_439-EYFP	Anion channelrhodopsin GcACR_439	Mammalian Expression	The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity. Sci Rep. 2017 Mar 3;7:43358. doi: 10.1038/srep43358.
	103143	pcDNA3.1-G1ACR_203-EYFP	Anion channelrhodopsin G1ACR_203	Mammalian Expression	The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity. Sci Rep. 2017 Mar 3;7:43358. doi: 10.1038/srep43358.
	103529	pcDNA3.1-PsuACR_433-EYFP	Anion channelrhodopsin PsuACR_433	Mammalian Expression	The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity. Sci Rep. 2017 Mar 3;7:43358. doi: 10.1038/srep43358.