Sriram Neelamegham Lab Materials

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• Plasmids
• Pooled Libraries
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Title	Authors	Publication
A GlycoGene CRISPR-Cas9 lentiviral library to study lectin binding and human glycan biosynthesis pathways.	Zhu Y, Groth T, Kelkar A, Zhou Y, Neelamegham S	Glycobiology. 2020 Aug 8. pii: 5889976. doi: 10.1093/glycob/cwaa074.
Conserved role of spike S2 domain N-glycosylation across betacoronaviruses	Yang Q, Kelkar A, Manicassamy B, Neelamegham S	npj Viruses. 2025 Jan; 3(4). doi: 10.1038/s44298-024-00085-7.
Detection of Plasma Protease Activity Using Microsphere-Cytometry Assays with E. coli Derived Substrates: VWF Proteolysis by ADAMTS13.	Gogia S, Lo CY, Neelamegham S	PLoS One. 2015 May 18;10(5):e0126556. doi: 10.1371/journal.pone.0126556. eCollection 2015.
Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing.	Kelkar A, Zhu Y, Groth T, Stolfa G, Stablewski AB, Singhi N, Nemeth M, Neelamegham S	Mol Ther. 2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 10.1016/j.ymthe.2019.09.006.
Escherichia coli-derived von Willebrand factor-A2 domain fluorescence/Forster resonance energy transfer proteins that quantify ADAMTS13 activity.	Dayananda KM, Gogia S, Neelamegham S	Anal Biochem. 2011 Mar 15;410(2):206-13. doi: 10.1016/j.ab.2010.12.005. Epub 2010 Dec 10.
Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan elaboration.	Yang Q, Hughes TA, Kelkar A, Yu X, Cheng K, Park S, Huang WC, Lovell JF, Neelamegham S	Elife. 2020 Oct 26;9. pii: 61552. doi: 10.7554/eLife.61552.
Tuning the tropism and infectivity of SARS-CoV-2 virus-like particles for mRNA delivery.	Yang Q, Davidson BA, Pajic P, Chen X, Gokcumen O, Gao M, Neelamegham S	Nucleic Acids Res. 2025 Feb 27;53(5):gkaf133. doi: 10.1093/nar/gkaf133.
Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion.	Stolfa G, Mondal N, Zhu Y, Yu X, Buffone A Jr, Neelamegham S	Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
von Willebrand factor self-association is regulated by the shear-dependent unfolding of the A2 domain.	Zhang C, Kelkar A, Neelamegham S	Blood Adv. 2019 Apr 9;3(7):957-968. doi: 10.1182/bloodadvances.2018030122.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
20.47.173050.164219	164219	ACE2	ACE2 (ACE2 Human)	Lentiviral	Inhibition of SARS-CoV-2 viral entry upon blocking N- and O-glycan elaboration. Elife. 2020 Oct 26;9. pii: 61552. doi: 10.7554/eLife.61552.
20.34.080754.124794	124794	pcDNA3.1-WT-VWF	VWF (VWF Human)	Mammalian Expression	von Willebrand factor self-association is regulated by the shear-dependent unfolding of the A2 domain. Blood Adv. 2019 Apr 9;3(7):957-968. doi: 10.1182/bloodadvances.2018030122.
	64847	pRSET-XS-VWF	Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Synthetic, Human)	Bacterial Expression	Escherichia coli-derived von Willebrand factor-A2 domain fluorescence/Forster resonance energy transfer proteins that quantify ADAMTS13 activity. Anal Biochem. 2011 Mar 15;410(2):206-13. doi: 10.1016/j.ab.2010.12.005. Epub 2010 Dec 10.
	64856	pRSET-A2-77p-Venus	VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)	Bacterial Expression	Detection of Plasma Protease Activity Using Microsphere-Cytometry Assays with E. coli Derived Substrates: VWF Proteolysis by ADAMTS13. PLoS One. 2015 May 18;10(5):e0126556. doi: 10.1371/journal.pone.0126556. eCollection 2015.
	80008	pX330-COSMC-KO	COSMC (C1GALT1C1 Human)	CRISPR	Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion. Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
	80009	pX330-MGAT1-KO	MGAT1 (MGAT1 Human)	CRISPR	Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion. Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
	80010	pX330-UGCG-KO	UGCG (UGCG Human)	CRISPR	Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion. Sci Rep. 2016 Jul 26;6:30392. doi: 10.1038/srep30392.
	124823	pcDNA3.1-deltaA1-VWF	VWF without A1 domain (VWF Human)	Mammalian Expression	von Willebrand factor self-association is regulated by the shear-dependent unfolding of the A2 domain. Blood Adv. 2019 Apr 9;3(7):957-968. doi: 10.1182/bloodadvances.2018030122.
	124824	pcDNA3.1-deltaA1-Lock-VWF	VWF without A1 domain and Lock A2 domain (VWF Human)	Mammalian Expression	von Willebrand factor self-association is regulated by the shear-dependent unfolding of the A2 domain. Blood Adv. 2019 Apr 9;3(7):957-968. doi: 10.1182/bloodadvances.2018030122.
	124825	pcDNA3.1-deltaA1-D1498A-VWF	VWF without A1 domain and D1498A mutation A2 domain (VWF Human)	Mammalian Expression	von Willebrand factor self-association is regulated by the shear-dependent unfolding of the A2 domain. Blood Adv. 2019 Apr 9;3(7):957-968. doi: 10.1182/bloodadvances.2018030122.

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	ID	Pooled Library	Vector Type	Publication
20.119.881877.140961	140961	Glycogene CRISPR Library	Lentiviral, CRISPR	A GlycoGene CRISPR-Cas9 lentiviral library to study lectin binding and human glycan biosynthesis pathways. Glycobiology. 2020 Aug 8. pii: 5889976. doi: 10.1093/glycob/cwaa074.