Jeffrey Chao Lab Materials

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Title	Authors	Publication
Live imaging of the co-translational recruitment of XBP1 mRNA to the ER and its processing by diffuse, non-polarized IRE1alpha.	Gomez-Puerta S, Ferrero R, Hochstoeger T, Zubiri I, Chao J, Aragon T, Voigt F	Elife. 2022 Jun 22;11. pii: 75580. doi: 10.7554/eLife.75580.
Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response.	Wilbertz JH, Voigt F, Horvathova I, Roth G, Zhan Y, Chao JA	Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006.
Single-Molecule Imaging Reveals Translation of mRNAs Localized to Stress Granules.	Mateju D, Eichenberger B, Voigt F, Eglinger J, Roth G, Chao JA	Cell. 2020 Dec 23;183(7):1801-1812.e13. doi: 10.1016/j.cell.2020.11.010. Epub 2020 Dec 11.
Single-molecule imaging reveals translation-dependent destabilization of mRNAs.	Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA	Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum.	Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, Chao JA, Palazzo AF	Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008.
The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells.	Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger J, Stadler MB, Giorgetti L, Chao JA	Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19.
Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.	Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer RH, Chao JA	Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.14.608990.119950	119950	phage UbiC G3BP1-GFP-GFP	G3BP1 (G3BP1 Human)	Mammalian Expression, Bacterial Expression, Lentiviral	Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response. Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006.
100.2.619238.64541	64541	phage UBC NLS-HA-2XMCP-tagRFPt	MCP	Mammalian Expression, Lentiviral	Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
100.2.042536.64542	64542	pmax pona 12xTRICK 24xMS2SL	12x PP7 stem-loops	Mammalian Expression	Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
50.1020.912302.104999	104999	phage UbiC NLS HA stdMCP stdHalo	stdMCP-stdHalo	Mammalian Expression, Bacterial Expression, Lentiviral	Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008.
50.1007.628713.119947	119947	phage UbiC tagRFP-T-DDX6	DDX6 (DDX6 Human)	Mammalian Expression, Bacterial Expression, Lentiviral	Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response. Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006.
50.3.964143.64540	64540	phage ubc nls ha 2xmcp HALO	MCP	Mammalian Expression, Lentiviral	Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
50.3.622902.64539	64539	phage ubc nls ha pcp gfp	PCP	Mammalian Expression, Lentiviral	Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
20.1010.636572.119945	119945	pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA	Renilla luciferase	Mammalian Expression, Bacterial Expression	Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response. Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006.
20.1010.151646.104998	104998	phage UbiC scAB-GFP	single chain Antibody against GCN4	Mammalian Expression, Bacterial Expression, Lentiviral	Single-Molecule Quantification of Translation-Dependent Association of mRNAs with the Endoplasmic Reticulum. Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 10.1016/j.celrep.2017.12.008.
20.7.644939.104099	104099	phage UbiC NLS HA stdPCP stdGFP	stdPCP-stdGFP	Mammalian Expression, Lentiviral	The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells. Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19.