Albert Jeltsch Lab Materials

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Title	Authors	Publication
Application of histone modification-specific interaction domains as an alternative to antibodies.	Kungulovski G, Kycia I, Tamas R, Jurkowska RZ, Kudithipudi S, Henry C, Reinhardt R, Labhart P, Jeltsch A	Genome Res. 2014 Nov;24(11):1842-53. doi: 10.1101/gr.170985.113. Epub 2014 Oct 9.
Application of mixed peptide arrays to study combinatorial readout of chromatin modifications.	Mauser R, Kungulovski G, Meral D, Maisch D, Jeltsch A	Biochimie. 2018 Mar;146:14-19. doi: 10.1016/j.biochi.2017.11.008. Epub 2017 Nov 11.
Application of recombinant TAF3 PHD domain instead of anti-H3K4me3 antibody.	Kungulovski G, Mauser R, Reinhardt R, Jeltsch A	Epigenetics Chromatin. 2016 Mar 22;9:11. doi: 10.1186/s13072-016-0061-9. eCollection 2016.
Discovery of NSD2 non-histone substrates and design of a super-substrate.	Weirich S, Kusevic D, Schnee P, Reiter J, Pleiss J, Jeltsch A	Commun Biol. 2024 Jun 8;7(1):707. doi: 10.1038/s42003-024-06395-z.
Engineering of Effector Domains for Targeted DNA Methylation with Reduced Off-Target Effects.	Hofacker D, Broche J, Laistner L, Adam S, Bashtrykov P, Jeltsch A	Int J Mol Sci. 2020 Jan 13;21(2). pii: ijms21020502. doi: 10.3390/ijms21020502.
Modular dual-color BiAD sensors for locus-specific readout of epigenome modifications in single cells.	Kohler AR, Hausser J, Harsch A, Bernhardt S, Haussermann L, Brenner LM, Lungu C, Olayioye MA, Bashtrykov P, Jeltsch A	Cell Rep Methods. 2024 Mar 26:100739. doi: 10.1016/j.crmeth.2024.100739.
Modular fluorescence complementation sensors for live cell detection of epigenetic signals at endogenous genomic sites.	Lungu C, Pinter S, Broche J, Rathert P, Jeltsch A	Nat Commun. 2017 Sep 21;8(1):649. doi: 10.1038/s41467-017-00457-z.
Protocol for Allele-Specific Epigenome Editing Using CRISPR/dCas9.	Rajaram N, Bashtrykov P, Jeltsch A	Methods Mol Biol. 2024;2842:179-192. doi: 10.1007/978-1-0716-4051-7_9.
Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity.	Siddique AN, Nunna S, Rajavelu A, Zhang Y, Jurkowska RZ, Reinhardt R, Rots MG, Ragozin S, Jurkowski TP, Jeltsch A	J Mol Biol. 2013 Feb 8;425(3):479-91. doi: 10.1016/j.jmb.2012.11.038. Epub 2012 Dec 4.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
50.0.572438.71827	71827	pET28-Dnmt3a3L-sc27	Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)	Bacterial Expression	Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity. J Mol Biol. 2013 Feb 8;425(3):479-91. doi: 10.1016/j.jmb.2012.11.038. Epub 2012 Dec 4.
	59694	pGEX-GP-2-MPHOSPH8 Chromo	MPHOSPH8 Chromo (MPHOSPH8 Human)	Bacterial Expression	Application of histone modification-specific interaction domains as an alternative to antibodies. Genome Res. 2014 Nov;24(11):1842-53. doi: 10.1101/gr.170985.113. Epub 2014 Oct 9.
	59695	pGEX-GP-2-CBX7 Chromo	CBX7 Chromo (CBX7 Human)	Bacterial Expression	Application of histone modification-specific interaction domains as an alternative to antibodies. Genome Res. 2014 Nov;24(11):1842-53. doi: 10.1101/gr.170985.113. Epub 2014 Oct 9.
	59696	pGEX-GP-2-DNMT3A PWWP	DNMT3A PWWP (Dnmt3a Mouse)	Bacterial Expression	Application of histone modification-specific interaction domains as an alternative to antibodies. Genome Res. 2014 Nov;24(11):1842-53. doi: 10.1101/gr.170985.113. Epub 2014 Oct 9.
	59697	pGEX-GP-2-CBX1 Chromo	CBX1 Chromo (CBX1 Human)	Bacterial Expression	Application of histone modification-specific interaction domains as an alternative to antibodies. Genome Res. 2014 Nov;24(11):1842-53. doi: 10.1101/gr.170985.113. Epub 2014 Oct 9.
	59698	pGEX-GP-2-ATRX ADD	ATRX ADD (ATRX Human)	Bacterial Expression	Application of histone modification-specific interaction domains as an alternative to antibodies. Genome Res. 2014 Nov;24(11):1842-53. doi: 10.1101/gr.170985.113. Epub 2014 Oct 9.
	59699	pGEX-GP-2-KDM4A double Tudor	KDM4A double Tudor (KDM4A Human)	Bacterial Expression	Application of histone modification-specific interaction domains as an alternative to antibodies. Genome Res. 2014 Nov;24(11):1842-53. doi: 10.1101/gr.170985.113. Epub 2014 Oct 9.
	92100	TAF3 PHD_pGEX-6P-2	TAF3 PHD domain (TAF3 Human)	Bacterial Expression	Application of recombinant TAF3 PHD domain instead of anti-H3K4me3 antibody. Epigenetics Chromatin. 2016 Mar 22;9:11. doi: 10.1186/s13072-016-0061-9. eCollection 2016.
	92101	BPTF PHD and Bromodomain	PHD and Bromodomain (BPTF Human)	Bacterial Expression	Application of mixed peptide arrays to study combinatorial readout of chromatin modifications. Biochimie. 2018 Mar;146:14-19. doi: 10.1016/j.biochi.2017.11.008. Epub 2017 Nov 11.
	154139	dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP	dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP	Mammalian Expression	Engineering of Effector Domains for Targeted DNA Methylation with Reduced Off-Target Effects. Int J Mol Sci. 2020 Jan 13;21(2). pii: ijms21020502. doi: 10.3390/ijms21020502.