Caixia Gao Lab Materials

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Title	Authors	Publication
An engineered prime editor with enhanced editing efficiency in plants.	Zong Y, Liu Y, Xue C, Li B, Li X, Wang Y, Li J, Liu G, Huang X, Cao X, Gao C	Nat Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w.
Discovery of deaminase functions by structure-based protein clustering.	Huang J, Lin Q, Fei H, He Z, Xu H, Li Y, Qu K, Han P, Gao Q, Li B, Liu G, Zhang L, Hu J, Zhang R, Zuo E, Luo Y, Ran Y, Qiu JL, Zhao KT, Gao C	Cell. 2023 Jul 20;186(15):3182-3195.e14. doi: 10.1016/j.cell.2023.05.041. Epub 2023 Jun 27.
Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A.	Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C	Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261.
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes.	Liang Z, Chen K, Li T, Zhang Y, Wang Y, Zhao Q, Liu J, Zhang H, Liu C, Ran Y, Gao C	Nat Commun. 2017 Jan 18;8:14261. doi: 10.1038/ncomms14261.
Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion.	Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C	Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
Gao Lab Plasmids	(unpublished)
Genome editing in plants with MAD7 nuclease.	Lin Q, Zhu Z, Liu G, Sun C, Lin D, Xue C, Li S, Zhang D, Gao C, Wang Y, Qiu JL	J Genet Genomics. 2021 May 3. pii: S1673-8527(21)00095-3. doi: 10.1016/j.jgg.2021.04.003.
High-efficiency prime editing with optimized, paired pegRNAs in plants.	Lin Q, Jin S, Zong Y, Yu H, Zhu Z, Liu G, Kou L, Wang Y, Qiu JL, Li J, Gao C	Nat Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: 10.1038/s41587-021-00868-w.
Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion.	Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C	Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors.	Sun C, Lei Y, Li B, Gao Q, Li Y, Cao W, Yang C, Li H, Wang Z, Li Y, Wang Y, Liu J, Zhao KT, Gao C	Nat Biotechnol. 2023 Apr 24. doi: 10.1038/s41587-023-01769-w.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.8.743170.98158	98158	pET28a-Cas9-His	NLS-Cas9-NLS	Bacterial Expression	Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes. Nat Commun. 2017 Jan 18;8:14261. doi: 10.1038/ncomms14261.
50.11.559196.183097	183097	pH-ePPE	Nls-nCas9-NC-nls-MLV-Nls	Plant Expression, CRISPR	An engineered prime editor with enhanced editing efficiency in plants. Nat Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w.
50.1.667330.98163	98163	pH-nCas9-PBE	NLS-APOBEC1-XTEN-nCas9-UGI-NLS	Plant Expression	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.
50.0.519015.53064	53064	pJIT163-2NLSCas9	Cas9		Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650.
20.12.812926.140448	140448	QPM-sgR (pTaU3)	TaU3-sgRNA	Plant Expression, CRISPR	Prime genome editing in rice and wheat. Nat Biotechnol. 2020 May;38(5):582-585. doi: 10.1038/s41587-020-0455-x. Epub 2020 Mar 16.
20.11.888389.213752	213752	U6-5'+3' MS2-CircRNA	5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme	Mammalian Expression, CRISPR	Prime editing using CRISPR-Cas12a and circular RNAs in human cells. Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02095-x.
20.2.454013.170131	170131	pH-nCas9-PPE-V2	nCas9(H840A)-M-MLV	Plant Expression, CRISPR	High-efficiency prime editing with optimized, paired pegRNAs in plants. Nat Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: 10.1038/s41587-021-00868-w.
20.2.043703.183095	183095	ePPE	Nls-nCas9-NC-nls-MLV-Nls	Plant Expression, CRISPR	An engineered prime editor with enhanced editing efficiency in plants. Nat Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w.
20.1.643000.183096	183096	ePPE-spG	Nls-nspGCas9-NC-nls-MLV-Nls	Plant Expression, CRISPR	An engineered prime editor with enhanced editing efficiency in plants. Nat Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w.
20.0.924120.98162	98162	pH-dCas9-PBE	NLS-APOBEC1-XTEN-dCas9-UGI-NLS	Plant Expression	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.