Emily Balskus Lab Materials

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Title	Authors	Publication
A metabolomics pipeline highlights microbial metabolism in bloodstream infections.	Mayers JR, Varon J, Zhou RR, Daniel-Ivad M, Beaulieu C, Bholse A, Glasser NR, Lichtenauer FM, Ng J, Vera MP, Huttenhower C, Perrella MA, Clish CB, Zhao SD, Baron RM, Balskus EP	Cell. 2024 Jun 11:S0092-8674(24)00579-8. doi: 10.1016/j.cell.2024.05.035.
A prodrug resistance mechanism is involved in colibactin biosynthesis and cytotoxicity.	Brotherton CA, Balskus EP	J Am Chem Soc. 2013 Mar 6;135(9):3359-62. doi: 10.1021/ja312154m. Epub 2013 Feb 20.
A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline.	Levin BJ, Huang YY, Peck SC, Wei Y, Martinez-Del Campo A, Marks JA, Franzosa EA, Huttenhower C, Balskus EP	Science. 2017 Feb 10;355(6325). pii: 355/6325/eaai8386. doi: 10.1126/science.aai8386.
An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin.	Ng TL, Rohac R, Mitchell AJ, Boal AK, Balskus EP	Nature. 2019 Feb;566(7742):94-99. doi: 10.1038/s41586-019-0894-z. Epub 2019 Feb 6.
Characterization of 1,2-Propanediol Dehydratases Reveals Distinct Mechanisms for B12-Dependent and Glycyl Radical Enzymes.	Levin BJ, Balskus EP	Biochemistry. 2018 Mar 16. doi: 10.1021/acs.biochem.8b00164.
Characterization of Polyketide Synthase Machinery from the pks Island Facilitates Isolation of a Candidate Precolibactin.	Zha L, Wilson MR, Brotherton CA, Balskus EP	ACS Chem Biol. 2016 May 20;11(5):1287-95. doi: 10.1021/acschembio.6b00014. Epub 2016 Mar 1.
Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring.	Zha L, Jiang Y, Henke MT, Wilson MR, Wang JX, Kelleher NL, Balskus EP	Nat Chem Biol. 2017 Oct;13(10):1063-1065. doi: 10.1038/nchembio.2448. Epub 2017 Aug 7.
Cylindrocyclophane biosynthesis involves functionalization of an unactivated carbon center.	Nakamura H, Hamer HA, Sirasani G, Balskus EP	J Am Chem Soc. 2012 Nov 14;134(45):18518-21. doi: 10.1021/ja308318p. Epub 2012 Nov 2.
Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.	Waldman AJ, Balskus EP	J Org Chem. 2018 May 29. doi: 10.1021/acs.joc.8b00367.
Discovery of small molecule protease inhibitors by investigating a widespread human gut bacterial biosynthetic pathway,	Schneider BA, EP Balskus	Tetrahedron

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
	45740	pET29b-NpR5599	NpR5599 (Npun_R5599 Nostoc punctiforme)	Bacterial Expression	The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2.
	45741	pET28b-NpR5600	NpR5600 (Npun_R5600 Nostoc punctiforme)	Bacterial Expression	The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2.
	45742	pET28b-Ava3856	Ava3856 (Ava_3856 Anabaena variabilis)	Bacterial Expression	The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2.
	45743	pET28b-Ava3855	Ava3855 (Ava_3855 Anabaena variabilis)	Bacterial Expression	The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2.
	45744	pET29b-Ava3858-3857	Ava3858-3857	Bacterial Expression	The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2.
	45745	pET29b-Ava3858-3856	Ava3858-3856	Bacterial Expression	The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2.
	45746	pET29b-Ava3858-3855	Ava3858-3855	Bacterial Expression	The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2.
	48198	pET-28a-CylA	CylA	Bacterial Expression	Cylindrocyclophane biosynthesis involves functionalization of an unactivated carbon center. J Am Chem Soc. 2012 Nov 14;134(45):18518-21. doi: 10.1021/ja308318p. Epub 2012 Nov 2.
	48199	pET-29b-CylB	CylB	Bacterial Expression	Cylindrocyclophane biosynthesis involves functionalization of an unactivated carbon center. J Am Chem Soc. 2012 Nov 14;134(45):18518-21. doi: 10.1021/ja308318p. Epub 2012 Nov 2.
	48200	pET-28a-CylI	CylI	Bacterial Expression	Cylindrocyclophane biosynthesis involves functionalization of an unactivated carbon center. J Am Chem Soc. 2012 Nov 14;134(45):18518-21. doi: 10.1021/ja308318p. Epub 2012 Nov 2.