James Inglese Lab Materials

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Title	Authors	Publication
A coincidence reporter-gene system for high-throughput screening.	Cheng KC, Inglese J	Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.
Active- and Allosteric-Site Cyclic Peptide Inhibitors of Secreted M. tuberculosis Chorismate Mutase.	van Neer RHP, Dranchak PK, Aitha M, Liu L, Carlson EK, Jacobsen IE, Battaile K, Fang Y, Tao D, Rai G, Padia J, Lovell S, Suga H, Inglese J	ACS Infect Dis. 2025 Mar 14;11(3):703-714. doi: 10.1021/acsinfecdis.4c00798. Epub 2025 Feb 4.
ICE1 promotes the link between splicing and nonsense-mediated mRNA decay.	Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg JR	Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178.
In vivo quantitative high-throughput screening for drug discovery and comparative toxicology.	Dranchak PK, Oliphant E, Queme B, Lamy L, Wang Y, Huang R, Xia M, Tao D, Inglese J	Dis Model Mech. 2023 Mar 1;16(3):dmm049863. doi: 10.1242/dmm.049863. Epub 2023 Mar 20.
Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression.	Auld DS, Thorne N, Maguire WF, Inglese J	Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3585-90. Epub 2009 Feb 10.
Methotrexate-based PROTACs as DHFR-specific chemical probes.	Rana S, Dranchak P, Dahlin JL, Lamy L, Li W, Oliphant E, Shrimp JH, Rajacharya GH, Tharakan R, Holland DO, Whitten AS, Wilson KM, Singh PK, Durum SK, Tao D, Rai G, Inglese J	Cell Chem Biol. 2024 Feb 15;31(2):221-233.e14. doi: 10.1016/j.chembiol.2023.09.014. Epub 2023 Oct 23.
Quantitative High-Throughput Screening Using a Coincidence Reporter Biocircuit.	Schuck BW, MacArthur R, Inglese J	Curr Protoc Neurosci. 2017 Apr 10;79:5.32.1-5.32.27. doi: 10.1002/cpns.27.
Serum-Stable and Selective Backbone-N-Methylated Cyclic Peptides That Inhibit Prokaryotic Glycolytic Mutases.	van Neer RHP, Dranchak PK, Liu L, Aitha M, Queme B, Kimura H, Katoh T, Battaile KP, Lovell S, Inglese J, Suga H	ACS Chem Biol. 2022 Aug 19;17(8):2284-2295. doi: 10.1021/acschembio.2c00403. Epub 2022 Jul 29.
Structure-activity relationship of ipglycermide binding to phosphoglycerate mutases.	Wiedmann M, Dranchak PK, Aitha M, Queme B, Collmus CD, Kashipathy MM, Kanter L, Lamy L, Rogers JM, Tao D, Battaile KP, Rai G, Lovell S, Suga H, Inglese J	J Biol Chem. 2021 Apr 1:100628. doi: 10.1016/j.jbc.2021.100628.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
50.4.007516.112085	112085	pKC-4.04	3XFLAG firefly luciferase-beta-globin (HBB Mouse, Photinus pyralis, Human)	Mammalian Expression	ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178.
50.3.592780.112084	112084	pKC-4.06	3XFLAG firefly luciferase-beta-globin 39PTC (HBB Mouse, Photinus pyralis, Human)	Mammalian Expression	ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178.
	41002	pCI-6.20	GSG-P2A-RLuc	Mammalian Expression, Luciferase	A coincidence reporter-gene system for high-throughput screening. Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.
	41003	pCI-6.24	GSG-P2A-RLuc, 4XCRE + Minimal promoter	Mammalian Expression, Luciferase	A coincidence reporter-gene system for high-throughput screening. Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.
	41045	pFLucWT	Firefly luciferase WT	Mammalian Expression, Luciferase	Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3585-90. Epub 2009 Feb 10.
	41046	pFLuc190UGA	Firefly luciferase 190UGA	Mammalian Expression	Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3585-90. Epub 2009 Feb 10.
	41047	pRLuc190UGA	RLuc190UGA	Mammalian Expression, Luciferase	Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3585-90. Epub 2009 Feb 10.
	41087	pCI-6.22	GSG-P2A-RLuc	Mammalian Expression, Luciferase	A coincidence reporter-gene system for high-throughput screening. Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170.
	41729	pRLuc110UGA	RLuc110UGA	Mammalian Expression, Luciferase	Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3585-90. Epub 2009 Feb 10.
	74229	pCI 9.0	3xFlag-FLuc-P2A-3xFlag-NLucPEST	Mammalian Expression	Quantitative High-Throughput Screening Using a Coincidence Reporter Biocircuit. Curr Protoc Neurosci. 2017 Apr 10;79:5.32.1-5.32.27. doi: 10.1002/cpns.27.