New England Biolabs

The following description was provided by New England Biolabs

Created "by scientists for scientists", New England Biolabs (NEB) has a focus on both basic and applied research and a culture that is both collaborative and academic. In support of open science, NEB has made many of their plasmids available to the research community through Addgene.

NEB is an industry leader in the discovery and production of enzymes for molecular biology applications and offers a large selection of recombinant and native enzymes for genomic research. NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, glycobiology, epigenetics and RNA analysis.

Resources

More information on New England Biolabs
Plasmid Collections Available:
- High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)
  - A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.
- New England Biolabs Cell-Imaging Plasmid Collection
  - Protein labeling systems based on SNAP-, CLIP-, ACP-, and MCP-tags
- Gene Addition Vectors
  - from Sibley MH, Raleigh EA.. 2012. A versatile element for gene addition in bacterial chromosomes. Nucleic Acids Res.. Feb;40(3):e19. PMID: 22123741
- MBP fusion plasmids
  - Maltose binding protein (MBP) is a common protein expression tag. Originally developed by NEB in the late 1980’s, it is one of the most well-known and accomplished means of tagging proteins expressed in microbes. Fusion of a target protein to MBP permits its one-step purification using amylose resin. Additionally, in E. coli, MBP is known to have significantly enhanced the solubility of many proteins it has been fused to.
  - The pMAL™ Protein Fusion and Purification System allows researchers to use E. coli to create a fusion between an expressed target protein and MBP, and purify the fusion protein in a single step. A gene of interest is cloned into a pMAL vector downstream of the malE gene that encodes MBP. The strong Ptac promoter and MBP translation initiation signals control expression of the gene. Upon induction, this system fuses the target protein sequence with a portion of MBP to create a fusion protein that is isolated using amylose affinity chromatography. Different pMAL vectors permit fusion proteins to be secreted into the periplasm or produced in the cytoplasm, and each introduce a protease cleavage site between MBP and the target protein to facilitate tag removal.
  - Learn more about MBP expression
- Snap-CBP fusion plasmids
  - New England Biolabs (NEB) has engineered a SNAP-CBD (chitin-binding domain) fusion protein that can be used in the preparation of chitin-binding probes. The labeling of SNAP-CBD with an appropriate SNAP-tag substrate is a highly efficient reaction that allows for consistent and effective labeling with good yields. The labeling procedure itself is robust and should be straightforward to conduct in most labs. Furthermore, the availability of SNAP-tag substrates bearing many different fluorophores increases the versatility of the SNAP-CBD protein by enabling the production of a wide variety of chitin-binding probes.
  - NEB developed a fusion construct comprised of Bacillus circulans CBD and a SNAP-tag protein to take advantage of the SNAP-tag labeling system, which allows for convenient and precise labeling with near-quantitative efficiency. With this strategy, one single fluorophore molecule reacts with the SNAP-tag portion of a fusion construct, thus avoiding the under- or over-labeling that is often observed with other chemical labeling techniques. Internal studies at NEB have shown that fluorescently-labeled SNAP-CBD is equally specific and efficient in detecting chitin in C. elegans as the earlier CBD-MBP probes. In addition, the availability of different SNAP-reactive fluorescent substrates as well as non-fluorescent substrates expands the utility of CBD in research. Many SNAP-tag-reactive substrates are commercially available from NEB.
Addgene Blog Posts featuring technologies from New England Biolabs: