Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.

Xie K, Minkenberg B, Yang Y
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
63142	pRGEB32	Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
63143	pGTR	PCR template to construct polycistronic tRNA-gRNA
63159	pRGE32	Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast