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Production of retroviral constructs for effective transfer and expression of T-cell receptor genes using Golden Gate cloning.

Coren LV, Jain S, Trivett MT, Ohlen C, Ott DE
Biotechniques. 2015 Mar 1;58(3):135-9. doi: 10.2144/000114265. eCollection 2015. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
64269MSGV Hu AcceptorRetroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes
64270MSGV Hu Acceptor PGK-NGFRRetroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFR
64271MSGV Rh AcceptorRetroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes
64272MSGV Rh Acceptor PGK-NGFRRetroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFR
128176MSGV Mouse AcceptorA TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9
128482MSGV Mouse Acceptor PGK NGFRA TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9

Antibodies from Article