CRISPR-Cas9-based Photoactivatable Transcription System.
Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64119 | NLS-dCas9-trCIB1 | Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1 |
| 64124 | NLSx3-CRY2PHR-p65 | Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain. |
| 64125 | GAL4UAS-Luciferase reporter | Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4. |
| 64126 | pNeurog2-Luciferase reporter | Photoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter. |
| 64127 | Tet-inducible Luciferase reporter | Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter. |
| 64128 | Tet-inducible mCherry reporter | Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter |
| 64129 | sgRNA1_ASCL1 | Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette. |
| 64130 | sgRNA2_ASCL1 | Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette. |
| 64131 | sgRNA3_ASCL1 | Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette. |
| 64132 | sgRNA4_ASCL1 | Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette. |
| 64136 | sgRNA1_MYOD1 | Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette. |
| 64137 | sgRNA2_MYOD1 | Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette. |
| 64138 | sgRNA3_MYOD1 | Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64139 | sgRNA4_MYOD1 | Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64140 | sgRNA1_IL1RN | Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64142 | sgRNA2_IL1RN | Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64151 | sgRNA3_IL1RN | Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64152 | sgRNA4_IL1RN | Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64153 | sgRNA1_NANOG | Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64154 | sgRNA2_NANOG | Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64155 | sgRNA3_NANOG | Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64156 | sgRNA4_NANOG | Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64157 | sgRNA1_GAL4UAS-Luciferase reporter | Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64158 | sgRNA2_GAL4UAS-Luciferase reporter | Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64159 | sgRNA3_GAL4UAS-Luciferase reporter | Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette |
| 64160 | sgRNA1_pNeurog2-Luciferase reporter | Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter. |
| 64161 | sgRNA1_Tet-inducible Luciferase reporter | Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter. |