Multicolor CRISPR labeling of chromosomal loci in human cells.
Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64104 | pHAGE-EFS-dCas9-GFP | Expression of Sp dCas9-GFP in mammalian cells |
| 64105 | pHAGE-SSFV-dCas9-GFP | Expression of Sp dCas9-GFP in mammalian cells |
| 64106 | pHAGE-TO-dCas9-GFP | Expression of Sp dCas9-GFP in mammalian cells |
| 64107 | pHAGE-TO-dCas9-3XGFP | Expression of Sp dCas9-3XGFP in mammalian cells |
| 64108 | pHAGE-TO-dCas9-3XmCherry | Expression of Sp dCas9-3XmCherry in mammalian cells |
| 64109 | pHAGE-TO-nmdCas9-3XGFP | Expression of Nm dCas9-3XGFP in mammalian cells |
| 64110 | pHAGE-TO-nmdCas9-3XmCherry | Expression of Nm dCas9-3XmCherry in mammalian cells |
| 64111 | pHAGE-TO-nls-st1dCas9-3nls-3XGFP | Expression of St1 dCas9-3XGFP in mammalian cells |
| 64112 | pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls | Expression of St1 dCas9-3XGFP in mammalian cells |
| 64113 | pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls | Expression of St1 dCas9-3XGFP in mammalian cells |
| 64114 | pLH-spsgRNA2 | Vector for expression of Sp sgRNA2 in mammalian cells |
| 64115 | pLH-nmsgRNA1.1 | Vector for expression of Nm sgRNA1.1 in mammalian cells |
| 64116 | pLH-stsgRNA1.1 | Vector for expression of St1 sgRNA1.1 in mammalian cells |
| 64117 | pLH-stsgRNA2.1 | Vector for expression of St1 sgRNA2.1 in mammalian cells |
| 64118 | pLH-stsgRNA3.1 | Vector for expression of St1 sgRNA3.1 in mammalian cells |
| 64512 | pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2 | Expression of St1 dCas9-3XTagBFP2 in mammalian cells |