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In vitro reconstitution of a highly processive recombinant human dynein complex.

Schlager MA, Hoang HT, Urnavicius L, Bullock SL, Carter AP
EMBO J. 2014 Sep 1;33(17):1855-68. doi: 10.15252/embj.201488792. Epub 2014 Jul 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
64067pDyn1Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).
64070GFP-BICD2NN-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)

Antibodies from Article