Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling.
Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W
Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64048 | pLenti-Lifeact-tdTomato | Fluorescent reporter for F-actin labeling in living cells |
| 64049 | pLenti-myc-GLUT4-mCherry | 3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation |
| 64050 | pLenti-FLAG-Akt2-CA | Myristoylated-FLAG-tagged Akt2, constitutively active |
| 70752 | pCMV5-HA-mTmod3-WT | N-terminal-HA-tagged mouse Tropomodulin3, WT |
| 70753 | pGEX-KG-mTmod3-WT | mouse Tropomodulin3, WT for bacterial expression |
| 70754 | pGEX-KG-mTmod3-1_163_domain | mouse Tropomodulin3, 1-163 domain for bacterial expression |
| 70755 | pGEX-KG-mTmod3-150-352_domain | mouse Tropomodulin3, 150-352 domain for bacterial expression |
| 70756 | pGEX-KG-mTmod3-1-325_domain | mouse Tropomodulin3, 1-325 domain for bacterial expression |