Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins.
Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear JE, Kuhlman B
Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 10.1073/pnas.1417910112. Epub 2014 Dec 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60408 | pQE-80L iLID (C530M) | E. coli expression of iLID (C530M) |
| 60409 | pQE-80L MBP-SspB Nano | E. coli expression of SspB Nano |
| 60410 | pQE-80L MBP-SspB Micro | E. coli expression of SspB Micro |
| 60411 | pLL7.0: Venus-iLID-CAAX (from KRas4B) | Mammalian Expression of Venus-iLID-CAAX |
| 60412 | pLL7.0: Venus-oLID-CAAX (from KRas4B) | Mammalian Expression of Venus-oLID-CAAX |
| 60413 | pLL7.0: Venus-iLID-Mito (From ActA) | Mammalian Expression of Venus-iLID-Mito |
| 60414 | pLL7.0: Venus-oLID-Mito (From ActA) | Mammalian Expression of Venus-oLID-Mito |
| 60415 | pLL7.0: tgRFPt-SSPB WT | Mammalian Expression of tgRFPt-Nano |
| 60416 | pLL7.0: tgRFPt-SSPB R73Q | Mammalian Expression of tgRFPt-Micro |
| 60418 | pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q | Mammalian Expression of Tiam DH/PH-tgRFPt-Micro |
| 60419 | pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT | Mammalian Expression of ITSN DH/PH-tgRFPt-Nano |
| 60420 | pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB R73Q | Mammalian Expression of ITSN DH/PH-tgRFPt-Micro |