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Addgene

A versatile modular vector system for rapid combinatorial mammalian genetics.

Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ
J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
62084pMuLE ENTR MCS L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
62085pMuLE ENTR MCS L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
62086pMuLE ENTR MCS R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
62087pMuLE ENTR MCS L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
62088pMuLE ENTR MCS L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
62089pMuLE ENTR MCS L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
62090pMuLE ENTR CMV L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62091pMuLE ENTR CMV L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62092pMuLE ENTR CMV L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62093pMuLE ENTR CMV R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62094pMuLE ENTR CMV loxP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site flanked by loxP sites. Compatible with MultiSite Gateway cloning
62095pMuLE ENTR SV40 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62096pMuLE ENTR SV40 L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62097pMuLE ENTR SFFV L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62098pMuLE ENTR SFFV L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62099pMuLE ENTR CMV/TO L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62100pMuLE ENTR CMV/TO R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
62101pMuLE ENTR H1 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62102pMuLE ENTR H1 L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62103pMuLE ENTR H1 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62104pMuLE ENTR H1 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62105pMuLE ENTR H1 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62106pMuLE ENTR 7SK L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62107pMuLE ENTR 7SK L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62108pMuLE ENTR 7SK L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62109pMuLE ENTR 7SK R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62110pMuLE ENTR 7SK L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62111pMuLE ENTR U6 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62112pMuLE ENTR U6 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
62113pMuLE ENTR U6-miR-30 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62114pMuLE ENTR U6-miR-30 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62115pMuLE ENTR U6-miR-30 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62116pMuLE ENTR U6-miR-30 L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62117pMuLE ENTR U6-miR-30 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62118pMuLE ENTR 7SK-miR-30 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62119pMuLE ENTR 7SK-miR-30 L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62120pMuLE ENTR 7SK-miR-30 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62121pMuLE ENTR 7SK-miR-30 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62122pMuLE ENTR CMV/TO-miR-30 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62123pMuLE ENTR CMV/TO-miR-30 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62124pMuLE ENTR CMV/TO-miR-30 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62125pMuLE ENTR CMV/TO-miR-30 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62126pMuLE ENTR CMV/TO-miR-30 L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62127pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
62128pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
62129pMuLE ENTR U6 stuffer sgRNA scaffold L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
62130pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
62131pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
62132pMuLE ENTR CMV-hCas9 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning
62133pMuLE ENTR SV40-hCas9 L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
62134pMuLE ENTR SV40-hCas9 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
62135pMuLE ENTR U6 n.s shRNA L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
62136pMuLE ENTR U6 n.s shRNA L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
62137pMuLE ENTR U6 n.s shRNA R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
62138pMuLE ENTR U6 n.s shRNA L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
62139pMuLE ENTR U6 n.s shRNA L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
62140pMuLE ENTR CMV eGFP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning
62141pMuLE ENTR CMV eGFP R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning
62142pMuLE ENTR CMV eGFP L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning
62143pMuLE ENTR CMV eGFP L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning
62144pMuLE ENTR SV40 eGFP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloning
62145pMuLE ENTR SV40 eGFP L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloning
62146pMuLE ENTR CMV mCherry L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning
62147pMuLE ENTR CMV mCherry R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning
62148pMuLE ENTR CMV mCherry L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning
62149pMuLE ENTR SV40 mCherry L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and mCherry module. Compatible with MultiSite Gateway cloning
62150pMuLE ENTR SV40 mCherry L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and mCherry module. Compatible with MultiSite Gateway cloning
62151pMuLE ENTR CMV tdTomato R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning
62152pMuLE ENTR CMV tdTomato L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning
62153pMuLE ENTR SV40 tdTomato L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and tdTomato module. Compatible with MultiSite Gateway cloning
62154pMuLE ENTR SV40 tdTomato L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and tdTomato module. Compatible with MultiSite Gateway cloning
62155pMuLE ENTR SFFV tdTomato L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and tdTomato module. Compatible with MultiSite Gateway cloning
62156pMuLE ENTR CMV iRFP L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning
62157pMuLE ENTR CMV iRFP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning
62158pMuLE ENTR CMV iRFP R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning
62159pMuLE ENTR CMV iRFP L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning
62160pMuLE ENTR SV40 iRFP L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and iRFP module. Compatible with MultiSite Gateway cloning
62161pMuLE ENTR CMV PuroR L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and Puromycin module. Compatible with MultiSite Gateway cloning
62162pMuLE ENTR CMV PuroR R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and Puromycin module. Compatible with MultiSite Gateway cloning
62163pMuLE ENTR CMV PuroR L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and Puromycin module. Compatible with MultiSite Gateway cloning
62164pMuLE ENTR SV40 PuroR L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning
62165pMuLE ENTR SV40 PuroR L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning
62166pMuLE ENTR CMV LacZ L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and LacZ module. Compatible with MultiSite Gateway cloning
62167pMuLE ENTR SV40 LacZ L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and LacZ module. Compatible with MultiSite Gateway cloning
62168pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning
62169pMuLE ENTR CMV Luc2 (FF-Luciferase) R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning
62170pMuLE ENTR SV40 Luc2 (FF-Luciferase) L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning
62171pMuLE ENTR SV40 Luc2 (FF-Luciferase) L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning
62172pMuLE ENTR CMV CreERT2 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning
62173pMuLE ENTR CMV CreERT2 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning
62174pMuLE ENTR SV40 CreERT2 L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and CreERT2 module. Compatible with MultiSite Gateway cloning
62175pMuLE Lenti Dest eGFPMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
62176pMuLE Lenti Dest iRFPMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
62177pMuLE Lenti Dest IFP1.4MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
62178pMuLE Lenti Dest NeoMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
62179pMuLE Lenti Dest Luc2MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses firefly luciferase under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
62180pMuLE ENTR 7SK-miR-30 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62181pMuLE ENTR H1-miR-30 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
62183pMuLE ENTR eGFP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing EGFP. Compatible with MultiSite Gateway cloning
62184pMuLE ENTR mCherry L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing mCherry. Compatible with MultiSite Gateway cloning
62185pMuLE ENTR tdTomato L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloning
62186pMuLE ENTR CMV Renilla Luciferase L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning

Antibodies from Article