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Synthetic Human Antibodies: Selecting and Evolving Functional Proteins Methods, A companion to Methods in Enzymology 8, 94-103

Barbas CF, Wagner J
Methods, A companion to Methods in Enzymology, 1995; 8(2): 94-103. (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
63900pComb3HTTBackbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
63901pAraH6HATTFor expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.
64133pComb3HSS2nd generation plasmid for phage display on modified geneIII, contains stuffer fragment

Antibodies from Article