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Methods for the generation of chicken monoclonal antibody fragments by phage display.

Andris-Widhopf J, Rader C, Steinberger P, Fuller R, Barbas CF 3rd
J Immunol Methods. 2000 Aug 28;242(1-2):159-81. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
63890pComb3XSS3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment
63891pComb3XTTBackbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
63892pComb3XLambdaBackbone pComb3X containing PCR template for human antibody lambda light chain constant region

Antibodies from Article