In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.
Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A
Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64071 | Adeno EA | Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4 |
| 64072 | Adeno Cas9 | Adenoviral vector for the expression of the Cas9 |
| 64073 | pX333 | Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter. |