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Structural Basis for the Activation and Inhibition of the UCH37 Deubiquitylase.

VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson H, Cohen RE, Yao T, Hill CP
Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 10.1016/j.molcel.2015.01.016. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
61928pET151-mUCH37 (ISF2)Expression of mUCH37 (ISF2) in E.coli
61929pET151-hUCH37 (ISF1)Expression of hUCH37 (ISF1) in E.coli
61930pET151-hUCH37 (ISF2)Expression of hUCH37 (ISF2) in E.coli
61931pET151-hUCH37 DMExpression of hUCH37 (ISF1) M269E/N291D in E.coli
61932pET151-hUCH37 (1-228)Expression of hUCH37 (1-228) in E.coli
61933pET151-hUCH37 (ISF1)(M148A/F149A)Expression of hUCH37 (M148A/F149A) in E.coli
61934pDEST15.1-hRPN13 (285-407)Expression of hRPN13 (285-407) in E.coli
61935pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli
61936pDEST15.1-hRPN13 (285-386)Expression of hRPN13 (285-386) in E.coli
61937pET3a-hUbExpression of hUb in E.coli
61938pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli
61939pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli
61940pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGSCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli
61941pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli
61943pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)Co-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coli
71388pET151-hUCH37 (ISF1)(M148D/F149D)Expression of hUCH37 (M148D/F149D) in E.coli

Antibodies from Article