A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations.
Bentley M, Decker H, Luisi J, Banker G
J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63569 | pBa-eGFP-flag-BicD2 594-FKBP | Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP |
| 64205 | pBa-tdTomato-flag-BicD2 594-FKBP | Bicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP |
| 64206 | pBa-flag-BicD2 594-FKBP | Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP |
| 64209 | pBa-FRB-3myc-Rab5a | Rab5a with N-terminal FRB and triple myc tags |
| 64210 | pBa-FRB-3myc-Rab7 | Rab7 with N-terminal FRB and triple myc tags |
| 64211 | pBa-KIF5C 559-tdTomato-FKBP | KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags |
| 64286 | pBa-FRB-3myc-KIF1A tail 391-1698 | KIF1A aa 391-1698 with N-terminal FRB and triple myc tags |
| 64287 | pBa-FRB-3myc-KIF1B beta tail 387-1770 | KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags |
| 64288 | pBa-FRB-3myc-KIF13A tail 361-1749 | KIF13A aa 361-1749 with N-terminal FRB and triple myc tags |
| 64289 | pBa-FRB-3myc-KIF13B tail 442-1826 | KIF13A aa 442-1826 with N-terminal FRB and triple myc tags |