Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry.
Meyer AJ, Ellefson JW, Ellington AD
ACS Synth Biol. 2014 Oct 7.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63622 | pQE-WT-CTGA-R13-AKSRV | CTGA-R13-AKSRV RNAP mutant |
| 63623 | pQE-WT-T3-R5-RRVH | T3-R5-RRVH RNAP mutant |
| 63624 | pQE-WT-KIF-R5-KIKR | KIF-R5-KIKR RNAP mutant |
| 63625 | pQE-WT-N4-R5-YRNRV | N4-R5-YRNRV RNAP mutant |
| 63626 | pQE-WT-CGG-R12-KIR | CGG-R12-KIR RNAP mutant |
| 63627 | pQE-WT-CTGA-R13-AKSIRV | CTGA-R13-AKSIRV RNAP mutant |
| 63628 | pQE-WT-T3-R5-RV | T3-R5-RV RNAP mutant |
| 63629 | pQE-WT-KIF-R5-IRH | K1F-R5-IRH RNAP mutant |
| 63630 | pQE-WT-N4-R5-RNRV | N4-R5-RNRV RNAP mutant |
| 63668 | pQE-WT-CGG-R12-KIRV | CGG-R12-KIRV RNAP mutant |