Fluorescent protein engineering by in vivo site-directed mutagenesis.

Valledor M, Hu Q, Schiller P, Myers RS
IUBMB Life. 2012 Aug;64(8):684-9. doi: 10.1002/iub.1041. Epub 2012 May 28. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
63215	pDual-eGFP	Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
63217	pDual-eGFP(W66)	Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
63218	pDual-eGFP(Stop66)	Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.
63558	pDual-eGFP(H66)	Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
63559	pDual-eGFP(Y203)	Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.