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Rational optimization of tolC as a powerful dual selectable marker for genome engineering.

Gregg CJ, Lajoie MJ, Napolitano MG, Mosberg JA, Goodman DB, Aach J, Isaacs FJ, Church GM
Nucleic Acids Res. 2014 Apr;42(7):4779-90. doi: 10.1093/nar/gkt1374. Epub 2014 Jan 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
64052EcM2.1Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling
64053EcM2.1.dtolCOptimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling
64054EcM2.1 dtolC bla::zeoROptimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

Antibodies from Article