Addgene: Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines.

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Eric Kowarz
Kowarz et al

Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines.

Kowarz E, Loescher D, Marschalek R
Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
60526	pSBbi-Bla	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene
60525	pSBbi-Neo	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene
60524	pSBbi-Hyg	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance gene
60523	pSBbi-Pur	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene
60522	pSBbi-RB	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene
60521	pSBbi-BB	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and blasticidin resistance gene
60520	pSBbi-GB	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene
60519	pSBbi-RN	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene
60518	pSBbi-BN	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene
60517	pSBbi-GN	SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene

Antibodies from Article