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SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties.

Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM
J Cell Sci. 2015 Jan 27. pii: jcs.162164. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
62967pBABEPuro-HA-GLI1Expresses HA-tagged Gli1
62970pLUT7 HA-GLI1Expresses HA-tagged Gli1
62972pcDNA3.1 HA-rnSOX9Expresses HA-tagged rat SOX9
62973pcDNA3.1 HA-rnSOX9 ΔC (1-381)Expresses HA-tagged rat SOX9 residues 1-383
62975pcDNA3.1 HA-rnSOX9 PQA/S (286-507)Expresses HA-tagged rat SOX9 residues 286-507
62976pcDNA3.1 myc-mβTrCP F-box (1-247)Expresses myc-tagged mouse βTrCP residues 1-247
62977pcDNA3.1 myc-mβTrCP WD1-7 (241-569)Expresses myc-tagged mouse βTrCP residues 241-569
62978pcDNA3.1 myc-mβTrCP WD2-7 (292-569)Expresses myc-tagged mouse βTrCP residues 292-569
63009pBABEPuro-rnSOX9Expresses rat SOX9

Antibodies from Article