Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension.
Karunarathne WK, Giri L, Kalyanaraman V, Gautam N
Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1565-74. doi: 10.1073/pnas.1220697110. Epub 2013 Mar 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62917 | CrBlue ---- pcDNA3.1 | CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity. |
| 62932 | Blue opsin mcherry ---- pcDNA3.1 | Blue opsin tagged with mcherry on the C terminus |
| 64203 | mcherry Gamma 9 ---pcDNA3.1 | G protein Gamma cone(9) subunit tagged with mcherry on the N terminus |
| 64204 | CrBlue-mcherry - pcDNA3.1 | CrBlue fused to mcherry on the C terminus |