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Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans.

Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus BG, Calidas D, Betzig E, Seydoux G
Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
62865pJW3.31Yeast two-hybrid bridge vector expressing PAA-1
62866pJW3.42Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion
62867pJW4.02Bacterial expression of MBP-PGL-1
62868pJW4.12Bacterial expression of MBP-PPTR-1
62869pJW4.13Bacterial expression of MBP-PPTR-2
62870pJW4.15Bacterial expression of MBP-PAA-1
62871pJW4.16Bacterial expression of MBP-MBK-2
62872pJW4.17Bacterial expression of MBP-MBK-2 K196R
62873pJW4.18Bacterial expression of MBP-MEI-1
62875pJW4.21Bacterial expression of MBP-MEG-1
62876pJW4.23Bacterial expression of MBP-MEG-3
62877pJW4.27Bacterial expression of MBP-MEG-1 S574A
62878pJW4.28Bacterial expression of MBP-MEG-3 T541A S582A T605A
62879pJW4.31Bacterial expression of GST-MEG-1
62880pJW4.33Bacterial expression of GST-MEG-3
62881pJW4.34Bacterial expression of GST-MEG-4
62882pJW6.01Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR
131369pJW4.33expression of GST:MEG-3 in C. elegans
131370pJW4.34expression of GST:MEG-4 in C. elegans
131371pJW4.31expression of GST:MEG-1 in C. elegans
131372pJW4.02expression of MBP:PGL-1 in C. elegans
131373pJW4.15expression of MBP:PAA-1 in C. elegans
131374pJW4.12expression of MBP:PPTR-1 in C. elegans
131375pJW4.13expression of MBP:PPTR-2 in C. elegans

Antibodies from Article