Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange.

Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
61020	GA-DEVD-GB-NES	green ddFP based caspase-3 biosensor
61019	RA-NES	ddRFP A-NES
61018	GA-NES	ddGFP A-NES
61017	GB-NES	ddGFP B-NES
60974	Ratiometric FPX sensor for ERK kinase activity	For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.
60973	RANLS-LEHD-BNES	Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
60972	RANLS-IETD-BNES	Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).
60937	pPHT-PIP2	Fluorescent FPX sensor for PIP2
60936	pPHT-PKA	Fluorescent FPX sensor for cAMP
60887	single polypeptide FPX biosensor for calcium ion	FPX biosensor